Invitrogen

beta-3 Tubulin Monoclonal Antibody (TU-20)

beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta-3 Tubulin Antibody (MA1-19187) in ICC/IF

Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 µg/mL) Monoclonal antibody (Product # MA1-19187). Nuclei were stained with DAPI (blue).

beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta-3 Tubulin Antibody in Western Blot (WB)

beta-3 Tubulin Antibody in Flow Cytometry (Flow)

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产品信息

MA1-19187

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL, 90 min

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

1-4 µg/mL

产品规格

种属反应

Human, Many, Mouse, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

TU-20

抗原

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_1070721

产品详细信息

This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

For immunohistochemistry applications, this product requires protein pretreatment (0.1% trypsin) in 0.1 M HCl; incubate for 30 minutes at room temperature.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Western Blot: Reducing conditions.

靶标信息

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Genomic, transcriptomic, and metabolomic profiles of hiPSC-derived dopamine neurons from clinically discordant brothers with identical PRKN deletions.

NPJ Parkinson's disease

Cukier HN,Kim H,Griswold AJ,Codreanu SG,Prince LM,Sherrod SD,McLean JA,Dykxhoorn DM,Ess KC,Hedera P,Bowman AB,Neely MD

MA1-19187 was used in Immunocytochemistry-immunoflourescence to identify genetic differences which could potentially, at least partially, contribute to the discordant clinical PD presentation of the two brothers.

Wed Jun 29 00:00:00 UTC 2022

Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach.

Marine drugs

Kirchhoff KN,Billion A,Voolstra CR,Kremb S,Wilke T,Vilcinskas A

MA1-19187 was used in Immunocytochemistry to develop a novel network pharmacology approach based on multi-omics functional data integration to predict how stingray venom disrupts the physiological systems of target animals.

Fri Dec 24 00:00:00 UTC 2021

Single cell RNA sequencing detects persistent cell type- and methylmercury exposure paradigm-specific effects in a human cortical neurodevelopmental model.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Diana Neely M,Xie S,Prince LM,Kim H,Tukker AM,Aschner M,Thimmapuram J,Bowman AB

MA1-19187 was used in Immunocytochemistry-immunofluorescence to demonstrate that MeHg results in changes in gene expression in human developing cortical neurons that manifest well after cessation of exposure and that these changes are cell type-, developmental stage-, and exposure paradigm-specific.

Sun Aug 01 00:00:00 UTC 2021

From the Cover: Manganese and Rotenone-Induced Oxidative Stress Signatures Differ in iPSC-Derived Human Dopamine Neurons.

Toxicological sciences : an official journal of the Society of Toxicology

Neely MD,Davison CA,Aschner M,Bowman AB

MA1-19187 was used in Immunocytochemistry-immunoflourescence to suggest that the cellular oxidative stress evoked by these 2 agents is distinct yielding unique oxidative stress signatures across outcome measures.

Sun Oct 01 00:00:00 UTC 2017

Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts.

Oncotarget

Eisch V,Lu X,Gabriel D,Djabali K

MA1-19187 was used in western blot test if progerin elicits spatiotemporal deviations in mitotic processes in Hutchinson-Gilford progeria syndrome fibroblasts

Tue Apr 26 00:00:00 UTC 2016

Novel crystalloid oligodendrogliopathy in hereditary spastic paraplegia.

Acta neuropathologica

Woehrer A,Laszlo L,Finsterer J,Stöllberger C,Furtner J,Rinner W,Molnar K,Budka H,Kovacs GG

MA1-19187 was used in immunohistochemistry to study crystalloid oligodendrogliopathy in hereditary spastic paraplegia

Mon Oct 01 00:00:00 UTC 2012

生物信息学

蛋白别名: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III

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基因别名: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4

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UniProt ID: (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4

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Entrez Gene ID: (Human) 10381, (Mouse) 22152, (Rat) 246118

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功能

GTPase activity structural constituent of cytoskeleton protein binding GTP binding peptide binding nucleotide binding

参与通路

microtubule-based process mitotic nuclear division axon guidance neuron differentiation protein polymerization metabolic process cytoskeleton organization