Invitrogen

beta-5 Tubulin Recombinant Rabbit Monoclonal Antibody (SR25-04)

beta-5 Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta-5 Tubulin Antibody (MA5-32008) in ICC/IF

Immunocytochemical analysis of beta-5 Tubulin in Hela cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

beta-5 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta-5 Tubulin Antibody in Western Blot (WB)

beta-5 Tubulin Antibody in Flow Cytometry (Flow)

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产品信息

MA5-32008

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000-1:20,000

免疫组化（石蜡） (IHC (P))

1:100-1:400

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:500-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SR25-04

抗原

Synthetic peptide within Human beta Tubulin aa 308-357

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809302

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tublin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. This antibody does not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. Ab-3 does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: beta Ib tubulin; tbb5; tubulin beta; Tubulin beta chain; tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, beta class I; tubulin, beta polypeptide

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基因别名: AA408537; AI596182; B130022C14Rik; CDCBM6; CSCSC1; M(beta)5; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB5

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UniProt ID: (Human) P07437, (Mouse) P99024, (Rat) P69897

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Entrez Gene ID: (Human) 203068, (Mouse) 22154, (Rat) 29214

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