Invitrogen

beta Amyloid Polyclonal Antibody

beta Amyloid Antibody in Immunocytochemistry (ICC/IF)

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beta Amyloid Antibody (36-6900) in ICC/IF

Immunofluorescence analysis of beta Amyloid was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta Amyloid Polyclonal Antibody (Product # 36-6900) at a concentration of 1 µg/mL in 0.1% BSA, incubated ... View More

beta Amyloid Antibody in Western Blot (WB)

beta Amyloid Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

beta Amyloid Antibody in Immunohistochemistry (Frozen) (IHC (F))

beta Amyloid Antibody in Western Blot, Immunohistochemistry (WB, IHC)

beta Amyloid Antibody in Immunohistochemistry (IHC)

beta Amyloid Antibody in Immunohistochemistry - Free Floating (IHC (Free))

beta Amyloid Antibody in Western Blot, Immunoprecipitation (WB, IP)

beta Amyloid Antibody in Immunoprecipitation (IP)

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产品信息

36-6900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看13篇已发表文章 13篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看7篇已发表文章 7篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Ferret, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the human and mouse amyloid-beta precursor protein (APP695)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533275

产品详细信息

For immunohistochemistry in formalin-fixed, paraffin-embedded tissues, digestion with trypsin prior to staining is required.

靶标信息

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activation of Wnt/β-catenin pathway mitigates blood-brain barrier dysfunction in Alzheimer's disease.

Brain : a journal of neurology

Wang Q,Huang X,Su Y,Yin G,Wang S,Yu B,Li H,Qi J,Chen H,Zeng W,Zhang K,Verkhratsky A,Niu J,Yi C

36-6900 was used in Immunohistochemistry-immunofluorescence to show dysfunctional blood-brain barrier in patients with Alzheimer's disease reflected by perivascular accumulation of blood-derived fibrinogen in the hippocampus and cortex, accompanied by decreased tight junction proteins Claudin-5 and glucose transporter Glut-1 in the brain endothelial cells.

Mon Dec 19 00:00:00 UTC 2022

Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients.

Biomedicines

Farré-Alins V,Palomino-Antolín A,Narros-Fernández P,Lopez-Rodriguez AB,Decouty-Perez C,Muñoz-Montero A,Zamorano-Fernández J,Mansilla-Fernández B,Giner-García J,García-Feijoo P,Sáez-Alegre M,Palpán-Flores AJ,Roda-Frade JM,Carabias CS,Rosa JM,Civantos-Martín B,Yus-Teruel S,Gandía L,Lagares A,Hernández-García BJ,Egea J

36-6900 was used in Immunohistochemistry (Frozen) to evaluate the individual and combined outcome prediction ability of SAA1 and TLR4, both related with inflammation and to compare them to S100β, a multiprotein that is found in astrocytes, used as biomarker for traumatic brain injury.

Tue May 25 00:00:00 UTC 2021

Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients.

Biomedicines

Tue May 25 00:00:00 UTC 2021

Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation.

Frontiers in immunology

Lennikov A,Saddala MS,Mukwaya A,Tang S,Huang H

36-6900 was used in Immunohistochemistry to show that with age, CXCR5 mice develop retinal degeneration characterised by microglia dysfunction, increased production of CXCL13 in the RPE progressive photoreceptor and neuronal loss.

Mon Oct 05 00:00:00 UTC 2020

Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation.

Frontiers in immunology

Lennikov A,Saddala MS,Mukwaya A,Tang S,Huang H

Mon Oct 05 00:00:00 UTC 2020

A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins.

Biological procedures online

Grant MKO,Shapiro SL,Ashe KH,Liu P,Zahs KR

36-6900 was used in Western Blotting to study two examples of antibody-independent artefacts that have confounded the interpretation of results in the search for molecular entities associated with memory loss in Alzheimer's disease.

Thu Oct 01 00:00:00 UTC 2020

Neuroprotective effect of dexmedetomidine in a murine model of traumatic brain injury.

Scientific reports

Wu J,Vogel T,Gao X,Lin B,Kulwin C,Chen J

36-6900 was used in Immunohistochemistry-immunofluorescence to investigate the effects of Dex on an animal model of traumatic brain injury.

Wed Mar 21 00:00:00 UTC 2018

Neuroprotective effect of dexmedetomidine in a murine model of traumatic brain injury.

Scientific reports

Wu J,Vogel T,Gao X,Lin B,Kulwin C,Chen J

36-6900 was used in Immunohistochemistry-immunofluorescence to investigate the effects of Dex on an animal model of traumatic brain injury.

Wed Mar 21 00:00:00 UTC 2018

Nimodipine treatment does not benefit juvenile ferrets with kaolin-induced hydrocephalus.

Fluids and barriers of the CNS

Di Curzio DL,Mao X,Baker A,Del Bigio MR

36-6900 was used in Immunohistochemistry to investigate whether treatment with nimodipine is beneficial to young ferrets with kaolin-induced hydrocephalus.

Thu May 03 00:00:00 UTC 2018

Repetitive Closed-Head Impact Model of Engineered Rotational Acceleration Induces Long-Term Cognitive Impairments with Persistent Astrogliosis and Microgliosis in Mice.

Journal of neurotrauma

Chen H,Desai A,Kim HY

36-6900 was used in Immunocytochemistry-immunoflourescence to investigate the long-term neuropathological and cognitive functional consequences of repeated mild traumatic brain injury.

Sat Jul 15 00:00:00 UTC 2017

Dendritic Spine Loss and Chronic White Matter Inflammation in a Mouse Model of Highly Repetitive Head Trauma.

The American journal of pathology

Winston CN,Noël A,Neustadtl A,Parsadanian M,Barton DJ,Chellappa D,Wilkins TE,Alikhani AD,Zapple DN,Villapol S,Planel E,Burns MP

36-6900 was used in immunohistochemistry - free floating to characterize chronic white matter inflammation and dendritic spine loss in a mouse model of highly repetitive head trauma

Tue Mar 01 00:00:00 UTC 2016

Quaternary Structure Defines a Large Class of Amyloid-β Oligomers Neutralized by Sequestration.

Cell reports

Liu P,Reed MN,Kotilinek LA,Grant MK,Forster CL,Qiang W,Shapiro SL,Reichl JH,Chiang AC,Jankowsky JL,Wilmot CM,Cleary JP,Zahs KR,Ashe KH

36-6900 was used in immunoprecipitation to classify subtypes of amyloid-beta oligomers

Tue Jun 23 00:00:00 UTC 2015

Hypersensitivity of the hippocampal CA3 region to stress-induced neurodegeneration and amyloidogenesis in a rat model of surgical menopause.

Brain : a journal of neurology

Zhang QG,Wang RM,Scott E,Han D,Dong Y,Tu JY,Yang F,Reddy Sareddy G,Vadlamudi RK,Brann DW

Published figure using beta Amyloid polyclonal antibody (Product # 36-6900) in Western Blot

Wed May 01 00:00:00 UTC 2013

Reduced subventricular zone proliferation and white matter damage in juvenile ferrets with kaolin-induced hydrocephalus.

Experimental neurology

Di Curzio DL,Buist RJ,Del Bigio MR

36-6900 was used in immunohistochemistry to characterize the behavioral, structural, and histological changes in juvenile ferrets following induction of hydrocephalus.

Tue Oct 01 00:00:00 UTC 2013

Memory deficits of British dementia knock-in mice are prevented by Aβ-precursor protein haploinsufficiency.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tamayev R,D'Adamio L

36-6900 was used in western blot to report that APP haploinsufficiency prevents memory dysfunctions seen in FBD(KI) mice.

Wed Apr 18 00:00:00 UTC 2012

Memory deficits of British dementia knock-in mice are prevented by Aβ-precursor protein haploinsufficiency.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tamayev R,D'Adamio L

36-6900 was used in western blot to report that APP haploinsufficiency prevents memory dysfunctions seen in FBD(KI) mice.

Wed Apr 18 00:00:00 UTC 2012

Critical role of NADPH oxidase in neuronal oxidative damage and microglia activation following traumatic brain injury.

PloS one

Zhang QG,Laird MD,Han D,Nguyen K,Scott E,Dong Y,Dhandapani KM,Brann DW

36-6900 was used in western blot to elucidate the role of NADPH oxidase in traumatic brain injury.

Fri Jul 27 00:00:00 UTC 2012

APP heterozygosity averts memory deficit in knockin mice expressing the Danish dementia BRI2 mutant.

The EMBO journal

Tamayev R,Matsuda S,Giliberto L,Arancio O,D'Adamio L

36-6900 was used in western blot to study APP/Bri2 complexes in a mouse model of familial Danish dementia.

Tue May 17 00:00:00 UTC 2011

Maturation of BRI2 generates a specific inhibitor that reduces APP processing at the plasma membrane and in endocytic vesicles.

Neurobiology of aging

Matsuda S,Matsuda Y,Snapp EL,D'Adamio L

36-6900 was used in western blot to elucidate how BRI2 regulates amyloid-beta precursor protein processing

Mon Aug 01 00:00:00 UTC 2011

Maturation of BRI2 generates a specific inhibitor that reduces APP processing at the plasma membrane and in endocytic vesicles.

Neurobiology of aging

Matsuda S,Matsuda Y,Snapp EL,D'Adamio L

36-6900 was used in western blot to elucidate how BRI2 regulates amyloid-beta precursor protein processing

Mon Aug 01 00:00:00 UTC 2011

Increased AβPP processing in familial Danish dementia patients.

Journal of Alzheimer's disease : JAD

Matsuda S,Tamayev R,D'Adamio L

36-6900 was used in western blot to test if AβPP processing is altered in Familial Danish Dementia patients' brain samples.

Thu Aug 16 00:00:00 UTC 2012

Increased AβPP processing in familial Danish dementia patients.

Journal of Alzheimer's disease : JAD

Matsuda S,Tamayev R,D'Adamio L

36-6900 was used in western blot to test if AβPP processing is altered in Familial Danish Dementia patients' brain samples.

Thu Aug 16 00:00:00 UTC 2012

Herpes simplex virus dances with amyloid precursor protein while exiting the cell.

PloS one

Cheng SB,Ferland P,Webster P,Bearer EL

36-6900 was used in immunocytochemistry to test if herpes simplex type 1 virus recruits cellular amyloid precursor protein during transport

Thu Mar 31 00:00:00 UTC 2011

Herpes simplex virus dances with amyloid precursor protein while exiting the cell.

PloS one

Cheng SB,Ferland P,Webster P,Bearer EL

36-6900 was used in immunocytochemistry to test if herpes simplex type 1 virus recruits cellular amyloid precursor protein during transport

Thu Mar 31 00:00:00 UTC 2011

BRI3 inhibits amyloid precursor protein processing in a mechanistically distinct manner from its homologue dementia gene BRI2.

The Journal of biological chemistry