Invitrogen

beta Arrestin 1 Polyclonal Antibody

View all (31) beta Arrestin 1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-87555

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 169-418 of human beta-arrestin1 (NP_0040322)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.85 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804249

Product Specific Information

Immunogen sequence: RKVQYAPERP GPQPTAETTR QFLMSDKPLH LEASLDKEIY YHGEPISVNV HVTNNTNKTV KKIKISVRQY ADICLFNTAQ YKCPVAMEEA DDTVAPSSTF CKVYTLTPFL ANNREKRGLA LDGKLKHEDT NLASSTLLRE GANREILGII VSYKVKVKLV VSRGGLLGDL ASSDVAVELP FTLMHPKPKE EPPHREVPEN ETPVDTNLIE LDTNDDDIVF EDFARQRLKG MKDDKEEEED GTGSPQLNNR; Positive Samples: U-937, K-562, Mouse brain, Mouse spleen; Cellular Location: Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, clathrin-coated pit, pseudopodium

Target Information

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: arrestin; arrestin 2; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1; beta-arrestin1; Non-visual arrestin-2

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Gene Aliases: 1200006I17Rik; ARB1; ARR1; ARRB1; AW208571; BARRES; G430100A01Rik

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UniProt ID: (Human) P49407, (Rat) P29066, (Mouse) Q8BWG8

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Entrez Gene ID: (Human) 408, (Rat) 25387, (Mouse) 109689

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Function(s)
histone acetyltransferase activity enzyme inhibitor activity GTPase activator activity insulin-like growth factor receptor binding protein binding transcription factor binding estrogen receptor binding mitogen-activated protein kinase kinase binding ubiquitin protein ligase binding alpha-1A adrenergic receptor binding alpha-1B adrenergic receptor binding angiotensin receptor binding follicle-stimulating hormone receptor binding V2 vasopressin receptor binding AP-2 adaptor complex binding clathrin adaptor activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process transcription regulatory region DNA binding ion channel binding protein phosphorylated amino acid binding G-protein coupled receptor binding clathrin binding phosphoprotein binding scaffold/adaptor protein
Process(es)
activation of MAPK activity positive regulation of protein phosphorylation G-protein coupled receptor internalization positive regulation of receptor internalization transcription from RNA polymerase II promoter apoptotic process phototransduction protein transport protein ubiquitination platelet activation negative regulation of protein ubiquitination positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity positive regulation of protein binding negative regulation of interleukin-6 production negative regulation of interleukin-8 production positive regulation of peptidyl-serine phosphorylation negative regulation of GTPase activity positive regulation of smooth muscle cell apoptotic process positive regulation of Rho protein signal transduction positive regulation of histone acetylation positive regulation of insulin secretion involved in cellular response to glucose stimulus response to antidepressant follicle-stimulating hormone signaling pathway stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of histone H4 acetylation negative regulation of protein phosphorylation desensitization of G-protein coupled receptor protein signaling pathway by arrestin endocytosis receptor-mediated endocytosis G-protein coupled receptor signaling pathway adenylate cyclase-modulating G-protein coupled receptor signaling pathway regulation of G-protein coupled receptor protein signaling pathway sensory perception of pain regulation of cAMP biosynthetic process regulation of cAMP catabolic process sensory perception of touch transcription, DNA-templated regulation of transcription, DNA-templated transport signal transduction negative regulation of signal transduction
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