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beta Galactosidase Fusion Protein Antibody (14-6773-81) in IHC
PLoS genetics 2018 -
Fig 6 Hyperactivity of P53 signaling in the NCC- mTOR KO mice. (A) Western blots for Mdm2, P53, P21 and Bax of E10.5 and E11.5 facial primordia. (B) Quantification of western blots of Mdm2, P53, P21 and Bax. (C, D) Immunofluorescence for P53 in the mandibular prominence of E10.5 mice. (E, F) Immunofluorescence for beta-galactosidase in the mandibular arch of E14.5 mice. Note, a number of positive cells are ... View More
产品信息
14-6773-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Bacteria
已发表种属
Bacteria,
Fruit fly,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with BSA, 0.02% gelatin
内含物
0.1% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_468331
产品详细信息
Description: The rabbit polyclonal antibody reacts with bBeta galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope.
Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.
Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Beta-Gal; betagal; bgal; ß-gal; ß-galactosidase; ßgal
