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AA Sequence of recombinant protein: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Betacellulin. Anti-Human Betacellulin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBetacellulin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Betacellulin (500-P254) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBetacellulin.
Western Blot: To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBetacellulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P254BT-1MG will be provided as 2 x 500 µg
The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: OTTHUMP00000160600; OTTHUMP00000219057; Probetacellulin
基因别名: BTC
UniProt ID: (Human) P35070
Entrez Gene ID: (Human) 685