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c-MAF Antibody (61-9855-42) in Flow
Th17-polarized BALB/c mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0042-82) and Mouse IgG2b kappa Isotype Control, PE-eFluor 610 (Product # 61-4732-80) (left) or c-MAF Monoclonal Antibody, PE-eFluor 610 (right). Viable cells in the lymphocytes gate were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
61-9855-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
sym0F1
抗原
Full-length E. coli-produced c-Maf
偶联物
激发/发射光谱
565/606 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2848502
产品详细信息
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Sym0F1 does not cross-react with MafA or MafB.
Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; c-Maf long form; c-maf proto-oncogene; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog
基因别名: 2810401A20Rik; A230108G15Rik; AW047063; AYGRP; c-MAF; CCA4; CTRCT21; MAF; Maf2
UniProt ID: (Human) O75444, (Mouse) P54843
Entrez Gene ID: (Human) 4094, (Mouse) 17132
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein homodimerization activity
protein heterodimerization activity
basic leucine zipper transcription factor
negative regulation of transcription from RNA polymerase II promoter
cytokine production
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
regulation of chondrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
inner ear development
lens fiber cell differentiation
lens development in camera-type eye
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
