Invitrogen

c-Rel Monoclonal Antibody (1RELAH5), PE, eBioscience™

图: 1 / 3

c-Rel Antibody (12-6111-80) in Flow

Mouse thymocytes were stained with Anti-Mouse CD4, Anti-Mouse CD8 and Anti-Mouse CD25, then fixed and permeabilized with Foxp3 Staining Buffer and stained intracellularly for Anti-Mouse Foxp3 and Anti-Mouse c-Rel PE. Anti-Mouse cRel PE staining (right) was compared in CD8+CD4. (left, green), CD4+CD8+ (left, blue), CD4+CD8-CD25-Foxp3. (center, orange), and CD4+CD8-CD25+Foxp3+ (center, pink) populations.

c-Rel Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

产品信息

12-6111-80

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1RELAH5

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11042978

产品详细信息

Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.

Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

12-6111-80 was used in Flow cytometry/Cell sorting to show that influenza infection rapidly induces EFRs, generating protective antibodies via Toll-like receptor (TLR)-mediated mechanisms that are both B cell intrinsic and extrinsic.

Wed Jul 05 00:00:00 UTC 2023

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Wed Jul 05 00:00:00 UTC 2023

NF-κB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells.

Nature immunology

Zhao M,Chauhan P,Sherman CA,Singh A,Kaileh M,Mazan-Mamczarz K,Ji H,Joy J,Nandi S,De S,Zhang Y,Fan J,Becker KG,Loke P,Zhou W,Sen R

12-6111-80 was used in Flow cytometry/Cell sorting to provide insights into exclusive functions of Rel and RelA in immunity and cancer.

Fri Sep 01 00:00:00 UTC 2023

A Regulatory Circuit Controlling the Dynamics of NFκB cRel Transitions B Cells from Proliferation to Plasma Cell Differentiation.

Immunity

Roy K,Mitchell S,Liu Y,Ohta S,Lin YS,Metzig MO,Nutt SL,Hoffmann A

12-6111-80 was used in Flow Cytometry to provide a mechanistic explanation of how dysregulation of this bi-stable circuit might result in pathologic B cell population phenotypes and thus offer new avenues for diagnostic stratification and treatment.

Tue Mar 19 00:00:00 UTC 2019

CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo.

Scientific reports

Ayre DC,Chute IC,Joy AP,Barnett DA,Hogan AM,Grüll MP,Peña-Castillo L,Lang AS,Lewis SM,Christian SL

12-6111 was used in Flow cytometry/Cell sorting to perform a systematic characterisation of B cell microvesicles released from WEHI-231 B lymphoma cells in response to CD24 stimulation.

Thu Aug 17 00:00:00 UTC 2017

A multi-scale approach reveals that NF-κB cRel enforces a B-cell decision to divide.

Molecular systems biology

Shokhirev MN,Almaden J,Davis-Turak J,Birnbaum HA,Russell TM,Vargas JA,Hoffmann A

12-6111 was used in Immunofluorescence to show that a multi-scale modeling approach allows for the prediction of dynamic organ-level physiology in terms of intra-cellular molecular networks.