Invitrogen

c-Rel Monoclonal Antibody (1RELAH5), eBioscience™

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c-Rel Antibody (14-6111-82) in WB

Western blot was performed using anti-c-REL Monoclonal Antibody(Product # 14-6111-82) and 65 kDa band was observed corresponding to c-REL across the Tissues tested. Tissue Extracts of (30 µg lysate) of Mouse Thymus (Lane 1),Mouse Spleen (Lane 2), Mouse Lung (Lane 3), Mouse Small Intestine (Lane 4) and Mouse Heart (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Bl... View More

c-Rel Antibody in Immunocytochemistry (ICC/IF)

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c-Rel Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of c-REL was observed in Mouse Thymus, Mouse spleen and Mouse Lung in comparison to no expression in Mouse Heart and Mouse Small Intestine using anti-c-REL Monoclonal Antibody(Product # 14-6111-82) in Western Blot. {RE}

产品信息

14-6111-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1RELAH5

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_11043473

产品详细信息

Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.

Applications Reported: This 1RELAH5 antibody has been reported for use in western blotting.

Applications Tested: This 1RELAH5 antibody has been tested by western blot analysis of mouse splenocytes. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using c-Rel monoclonal antibody (Product # 14-6111-82) in Flow Cytometry

Wed Jul 05 00:00:00 UTC 2023

Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b.

Nature communications

Cho S,Lee HM,Yu IS,Choi YS,Huang HY,Hashemifar SS,Lin LL,Chen MC,Afanasiev ND,Khan AA,Lin SW,Rudensky AY,Crotty S,Lu LF

Published figure using c-Rel monoclonal antibody (Product # 14-6111-82) in Western Blot

Mon Jul 16 00:00:00 UTC 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

14-6111 was used in Western Blotting to study how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements.

Wed Sep 19 00:00:00 UTC 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

14-6111 was used in Western Blotting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 UTC 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

14-6111 was used in Western Blotting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 UTC 2017

A multi-scale approach reveals that NF-κB cRel enforces a B-cell decision to divide.

Molecular systems biology

Shokhirev MN,Almaden J,Davis-Turak J,Birnbaum HA,Russell TM,Vargas JA,Hoffmann A

Published figure using c-Rel monoclonal antibody (Product # 14-6111-82) in Flow Cytometry

Fri Feb 13 00:00:00 UTC 2015

Cutting Edge: Synchronization of IRF1, JunB, and C/EBPβ Activities during TLR3-TLR7 Cross-Talk Orchestrates Timely Cytokine Synergy in the Proinflammatory Response.

Journal of immunology (Baltimore, Md. : 1950)

Liu Q,Zhu Y,Yong WK,Sze NS,Tan NS,Ding JL

14-6111 was used in Western Blotting to investigate the detailed mechanisms of multiple pathogen-associated molecular pattern-induced TLR pathway cross-talk.