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eIF1AX Antibody (MA5-44706) in WB
Western blot analysis of eIF1A in HEK-293T cell lysate (10 µg/Lane). Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in eIF1A Monoclonal antibody (Product # MA5-44706) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Predicted band size: 16 kDa. Observed band size: 18 kDa.
产品信息
MA5-44706
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE64-52
抗原
Synthetic peptide within human eIF1A aa 71-120/144.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2931164
靶标信息
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-1A X isoform; eIF-4C; eukaryotic translation initiation factor 1A, X chromosome; Eukaryotic translation initiation factor 1A, X-chromosomal; Eukaryotic translation initiation factor 4C; Putative eukaryotic translation initiation factor 1A
基因别名: eIF-1A; eIF-4C; EIF1A; EIF1AP1; EIF1AX; EIF4C
UniProt ID: (Human) P47813
Entrez Gene ID: (Human) 1964
