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eIF2 gamma Antibody (PA5-92081) in WB
Western blot analysis of eIF2 gamma in extracts of Rat brain. Samples were incubated with eIF2 gamma Polyclonal antibody (Product # PA5-92081) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-92081
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_0014061)
偶联物
形式
Liquid
浓度
0.26 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2806375
产品详细信息
Immunogen sequence: HLAAIEIMKL KHILILQNKI DLVKESQAKE QYEQILAFVQ GTVAEGAPII PISAQLKYNI EVVCEYIVKK IPVPPRDFTS EPRLIVIRSF DVNKPGCEVD DLKGGVAGGS ILKGVLKVGQ EIEVRPGIVS KDSEGKLMCK PIFSKIVSLF AEHNDLQYAA PGGLIGVGTK IDPTLCRADR MVGQVLGAVG ALPEIFTELE ISYFLLRRLL GVRTEGDKKA AKVQKLSKNE VLMVNIGSLS TGGRVSAVKA DLGKIVLTNP VCTEVGEKIA LSRRVEKHWR LIGWGQIRRG VTIKPTVDDD; Positive Samples: Rat brain
靶标信息
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2 alpha, eIF2 beta and eIF2 gamma (eukaryotic translation initiation factor 2 alpha, beta and gamma, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2S3 (eukaryotic translation initiation factor 2, subunit 3), also known as eIF2G, is a 472 amino acid protein that belongs to the gamma subfamily of GTP-binding elongation factor proteins. Existing as a heterotrimer composed of an alpha, beta and gamma chain, eIF2S3 functions to bind ribosomal subunits and catalyze the subsequent formation of preinitiation complexes necessary for protein synthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-2-gamma; eIF2-gamma; eukaryotic translation initiation factor 2 subunit 3; Eukaryotic translation initiation factor 2 subunit 3, X-linked; Eukaryotic translation initiation factor 2 subunit gamma; Eukaryotic translation initiation factor 2 subunit gamma, X-linked; PP42
基因别名: Eif2g; Eif2s3; Eif2s3x; PP42
UniProt ID: (Rat) P81795
Entrez Gene ID: (Rat) 299027
