Invitrogen

eIF2b alpha Polyclonal Antibody

eIF2b alpha Antibody in Immunocytochemistry (ICC/IF)

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eIF2b alpha Antibody (PA5-28992) in ICC/IF

eIF2b alpha Polyclonal Antibody detects EIF2B1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2B1 protein stained by eIF2b alpha Polyclonal Antibody (Product # PA5-28992) diluted at 1:500. Blue: Hoechst 33342 staining.

eIF2b alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

eIF2b alpha Antibody in Western Blot (WB)

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产品信息

PA5-28992

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human EIF2B1 Recombinant protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546468

产品详细信息

Recommended positive controls: 293T, A431, Jurkat, Raji.

Predicted reactivity: Mouse (94%), Rat (92%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

EIF2B2 mutations in vanishing white matter disease hypersuppress translation and delay recovery during the integrated stress response.

RNA (New York, N.Y.)

Moon SL,Parker R

PA5-28992 was used in Western Blotting to demonstrate that small molecules targeting eIF2B or the eIF2α kinase PERK rescue translation defects in patient cells.

Fri Jun 01 00:00:00 UTC 2018

EIF2B2 mutations in vanishing white matter disease hypersuppress translation and delay recovery during the integrated stress response.

RNA (New York, N.Y.)

Moon SL,Parker R

PA5-28992 was used in Western Blotting to demonstrate that small molecules targeting eIF2B or the eIF2α kinase PERK rescue translation defects in patient cells.

Fri Jun 01 00:00:00 UTC 2018

生物信息学

蛋白别名: eIF-2B GDP-GTP exchange factor subunit alpha; eIF2B GDP-GTP exchange factor subunit alpha; eukaryotic translation initiation factor 2B, subunit 1 (alpha, 26kDa); eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa; translation initiation factor eIF-2B subunit alpha; Translation initiation factor eIF2B subunit alpha

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基因别名: 26kDa; D5Ertd406e; EIF2B; EIF2B1; EIF2BA

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UniProt ID: (Human) Q14232, (Mouse) Q99LC8

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Entrez Gene ID: (Human) 1967, (Mouse) 209354

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功能

translation initiation factor activity guanyl-nucleotide exchange factor activity protein binding S-methyl-5-thioribose-1-phosphate isomerase activity GTP binding GDP binding enzyme regulator activity translation initiation factor

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