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eIF4A2 Antibody (PA5-79195) in ICC/IF
Immunocytochemistry analysis of eIF4A2 using anti-eIF4A2 antibody (Product # PA5-79195) . eIF4A2 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-eIF4A2 antibody (Product # PA5-79195) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAP... View More
产品信息
PA5-79195
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2 (5-31aa SADYNREHGGPEGMDPDGVIESNWNEI).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2746311
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; eIF-4A-II; eIF4A-II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; eukaryotic translation initiation factor 4A, isoform 2; N-terminally processed; protein synthesis initiation factor 4AII
基因别名: 4833432N07Rik; BM-010; DDX2B; eIF-4A-II; Eif4; EIF4A; eIF4A-II; EIF4A2; EIF4F
UniProt ID: (Human) Q14240, (Mouse) P10630, (Rat) Q5RKI1
Entrez Gene ID: (Human) 1974, (Mouse) 13682, (Rat) 303831
