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eRF1 Antibody (PA5-28777) in ICC/IF
Immunofluorescence analysis of eRF1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with eRF1 Rabbit Polyclonal Antibody(Product # PA5-28777) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
产品信息
PA5-28777
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a region within amino acids 374 and 437 of eRF1
偶联物
形式
Liquid
浓度
0.91 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546253
产品详细信息
Recommended positive controls: Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eRF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; polypeptide chain release factor 1; Protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; TB3-1
基因别名: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; RF1; SUP45L1; TB3-1
UniProt ID: (Human) P62495, (Mouse) Q8BWY3, (Rat) Q5U2Q7
Entrez Gene ID: (Human) 2107, (Mouse) 225363, (Rat) 307503
