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eRF1 Antibody (PA5-28777) in ICC/IF
Immunofluorescence analysis of eRF1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with eRF1 Rabbit Polyclonal Antibody(Product # PA5-28777) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
PA5-28777
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 374 and 437 of eRF1
Conjugate
Form
Liquid
Concentration
0.91 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546253
Product Specific Information
Recommended positive controls: Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eRF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; polypeptide chain release factor 1; Protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; TB3-1
Gene Aliases: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; RF1; SUP45L1; TB3-1
UniProt ID: (Human) P62495, (Mouse) Q8BWY3, (Rat) Q5U2Q7
Entrez Gene ID: (Human) 2107, (Mouse) 225363, (Rat) 307503
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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