Invitrogen

hnRNP F Polyclonal Antibody

hnRNP F Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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hnRNP F Antibody (PA5-22341) in IHC (P)

hnRNP F Polyclonal Antibody detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis. Sample: Paraffin-embedded mouse uterus. HnRNP F Polyclonal Antibody (Product # PA5-22341) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

hnRNP F Antibody in Immunoprecipitation (IP)

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产品信息

PA5-22341

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 204 of hnRNP F (Uniprot ID#P52597)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.45 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155780

产品详细信息

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (98%), Rat (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The RNA-binding protein hnRNP F is required for the germinal center B cell response.

Nature communications

Huang H,Li Y,Zhang G,Ruan GX,Zhu Z,Chen W,Zou J,Zhang R,Wang J,Ouyang Y,Xu S,Ou X

PA5-22341 was used in Western Blot to find that hnRNP A1 and A2B1 can bind to the same region of Cd40 pre-mRNA but suppress exon 6 inclusion, suggesting that these hnRNPs and hnRNP F might antagonize each-other's effects on Cd40 splicing.

Thu Mar 30 00:00:00 UTC 2023

The RNA-binding protein hnRNP F is required for the germinal center B cell response.

Nature communications

Huang H,Li Y,Zhang G,Ruan GX,Zhu Z,Chen W,Zou J,Zhang R,Wang J,Ouyang Y,Xu S,Ou X

Thu Mar 30 00:00:00 UTC 2023

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export.

Cell reports

Duan L,Zaepfel BL,Aksenova V,Dasso M,Rothstein JD,Kalab P,Hayes LR

PA5-22341 was used in Immunocytochemistry-immunofluorescence to show that TDP-43 exits nuclei by passive diffusion, independent of facilitated mRNA export.