Invitrogen

nNOS Polyclonal Antibody

11个已发表图片
20 篇参考文献
查看其他18个nNOS抗体
说明书
实验方案
技术支持
说明书
实验方案
技术支持

产品信息

PA3-032A

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

-
查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:400
查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

1:800
查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:250
查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

Assay-dependent
查看2篇已发表文章 2篇已发表文章

其他PubMed (MISC)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Bovine, Human, Mouse, Rabbit, Rat

已发表种属

Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.
查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2236017

产品详细信息

PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).

PA3-032A has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract. Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.

The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.

靶标信息

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: BNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

查看更多 收起

基因别名: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1

查看更多 收起

UniProt ID: (Human) P29475, (Rabbit) O19132, (Rat) P29476, (Mouse) Q9Z0J4

查看更多 收起

Entrez Gene ID: (Human) 4842, (Rabbit) 100009243, (Rat) 24598, (Mouse) 18125

查看更多 收起

功能
nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding NADPH-hemoprotein reductase activity amino acid binding ATPase binding
参与通路
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process muscle contraction striated muscle contraction nitric oxide mediated signal transduction female pregnancy aging regulation of heart contraction negative regulation of cell proliferation response to heat response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to nutrient levels response to lipopolysaccharide negative regulation of peptidyl-serine phosphorylation response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport myoblast fusion negative regulation of calcium ion transport into cytosol neurotransmitter biosynthetic process cell redox homeostasis regulation of neurogenesis regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity negative regulation of adrenergic receptor signaling pathway involved in heart process regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of cardiac conduction
guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持