Invitrogen

p16INK4a-TAT Polyclonal Antibody, Biotin, PeproTech®

p16INK4a-TAT Antibody in Western Blot (WB)

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p16INK4a-TAT Antibody (500-P284TBT-25UG) in WB

Western Blot: To detect Human p16-INK4a-TAT by Western Blot analysis, p16INK4a-TAT Polyclonal Antibody, Biotin (Product # 500-P284TBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P284TBT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 18.0 kDa Recombinant Human p16-INK4a-TAT

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930239

产品详细信息

AA Sequence of recombinant protein: EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human p16-INK4a-TAT by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody,in conjunction with PeproTech Polyclonal Anti-Human p16-INK4a-TAT (500-P284) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human p16-INK4a-TAT.

Western Blot: To detect Human p16-INK4a-TAT by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL.When used in conjunction with compatible development reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P284TBT-1MG will be provided as 2 x 500 µg

靶标信息

p16-INK4a is a nuclear protein that regulates the cell cycle by inhibiting cyclin-dependent kinase-4 (CDK4) and CDK6. p16-INK4a inhibits CDK activity by binding to the CDK molecules in a manner that interferes with their ability to interact with cyclin D. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of p16-INK4a steadily increases with age, and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the p16-INK4a gene is frequently found in melanomas, as well as in certain other types of cancer. p16-INK4a and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary, as well as transformed, cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; cell cycle negative regulator beta; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); MTS-1; Multiple tumor suppressor 1; p14ARF; p16-INK4; p16-INK4a; p16INK4a; Tumor suppressor ARF

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基因别名: ARF; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16

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UniProt ID: (Human) P42771

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Entrez Gene ID: (Human) 1029

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功能

cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding protein kinase binding poly(A) RNA binding NF-kappaB binding

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