Invitrogen

p21 (WAF1, Cip1) Polyclonal Antibody, eBioscience™

p21 (WAF1, Cip1) Antibody in Immunocytochemistry (ICC/IF)

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p21 (WAF1, Cip1) Antibody (14-6715-81) in ICC/IF

Immunofluorescence analysis of p21 (WAF1,Cip1) was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p21 (WAF1,Cip1) Rabbit Polyclonal Antibody (Product # 14-6715-81) at 5 microgram/mL in 0.1% BSA, incubated... View More

p21 (WAF1, Cip1) Antibody in Western Blot (WB)

p21 (WAF1, Cip1) Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

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产品信息

14-6715-81

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

Assay-Dependent

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免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

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流式细胞分析 (Flow)

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免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468227

产品详细信息

Description: The polyclonal antibody reacts with mouse, rat, and human p21; the antibody was raised against a peptide mapping to the carboxy terminus of human p21. The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

Applications Reported: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been tested by immunoblotting (WB) (1:200 starting dilution).

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183.

Scientific reports

Di Luccia B,Acampora V,Saggese A,Calabrò V,Vivo M,Angrisano T,Baccigalupi L,Ricca E,Pollice A

14-6715-81 was used in Western Blotting to show that bioactive molecules secreted by SF1183 reduce the proliferation of HCT116 cells in a reversible manner determining a variation in cell cycle markers (p21WAF, p53, cyclin D1) and resulting in the protection of HCT116 cells from TNF-alfa induced apoptosis, an effect potentially relevant for the protection of the epithelial barrier integrity and reconstitution of tissue homeostasis.

Thu Nov 24 00:00:00 UTC 2022

Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183.

Scientific reports

Di Luccia B,Acampora V,Saggese A,Calabrò V,Vivo M,Angrisano T,Baccigalupi L,Ricca E,Pollice A

Thu Nov 24 00:00:00 UTC 2022

Targeting p21^Cip1 highly expressing cells in adipose tissue alleviates insulin resistance in obesity.

Cell metabolism

Wang L,Wang B,Gasek NS,Zhou Y,Cohn RL,Martin DE,Zuo W,Flynn WF,Guo C,Jellison ER,Kim T,Prata LGPL,Palmer AK,Li M,Inman CL,Barber LS,Al-Naggar IMA,Zhou Y,Du W,Kuchel GA,Meves A,Tchkonia T,Kirkland JL,Robson P,Xu M

14-6715-81 was used in Flow cytometry/Cell sorting to establish that p21high cells within fat are sufficient to cause insulin resistance in vivo.

Tue Jan 04 00:00:00 UTC 2022

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells.

Nature communications

Jia W,Kong L,Kidoya H,Naito H,Muramatsu F,Hayashi Y,Hsieh HY,Yamakawa D,Hsu DK,Liu FT,Takakura N

Published figure using p21 (WAF1, Cip1) polyclonal antibody (Product # 14-6715-81) in Immunocytochemistry

Fri Apr 09 00:00:00 UTC 2021

Sympathetic Denervation Ameliorates Renal Fibrosis via Inhibition of Cellular Senescence.

Frontiers in immunology

Li Q,Deng Y,Liu L,Zhang C,Cai Y,Zhang T,Han M,Xu G

14-6715-81 was used in Western Blotting to test the hypothesis that sympathetic activation in the injured kidney induces cellular senescence, which contributes to progressive renal inflammation and fibrosis.

Thu Mar 03 00:00:00 UTC 2022

Sympathetic Denervation Ameliorates Renal Fibrosis via Inhibition of Cellular Senescence.

Frontiers in immunology

Li Q,Deng Y,Liu L,Zhang C,Cai Y,Zhang T,Han M,Xu G

Thu Mar 03 00:00:00 UTC 2022

Upregulation of Chemoresistance by Mg²⁺ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.

Cells

Onuma S,Manabe A,Yoshino Y,Matsunaga T,Asai T,Ikari A

14-6715-81 was used in Western Blot to investigate the effect of low Mg2+ concentration (LM) on proliferation and chemosensitivity using human lung adenocarcinoma A549 cells.

Wed May 12 00:00:00 UTC 2021

Upregulation of Chemoresistance by Mg²⁺ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.

Cells

Onuma S,Manabe A,Yoshino Y,Matsunaga T,Asai T,Ikari A

14-6715-81 was used in Western Blot to investigate the effect of low Mg2+ concentration (LM) on proliferation and chemosensitivity using human lung adenocarcinoma A549 cells.

Wed May 12 00:00:00 UTC 2021

An inducible p21-Cre mouse model to monitor and manipulate p21-highly-expressing senescent cells in vivo.

Nature aging

Wang B,Wang L,Gasek NS,Zhou Y,Kim T,Guo C,Jellison ER,Haynes L,Yadav S,Tchkonia T,Kuchel GA,Kirkland JL,Xu M

14-6715-81 was used in Immunocytochemistry-immunofluorescence to demonstrate that the p21-Cre mouse model is a valuable and powerful tool for studying p21 high cells to further understand the biology of senescent cells.

Fri Oct 01 00:00:00 UTC 2021

CRISPR/Cas9 treatment causes extended TP53-dependent cell cycle arrest in human cells.

Nucleic acids research

Geisinger JM,Stearns T

14-6715-63 was used in Immunocytochemistry to test a hypothesis that the basis for the large variation in mutant recovery for a given target sequence between cell lines may be due to differences in how the DNA damage response affects cell cycle progression.

Fri Sep 18 00:00:00 UTC 2020

Delayed effects of acute whole body lethal radiation exposure in mice pre-treated with BBT-059.

Scientific reports

Sharma NK,Holmes-Hampton GP,Kumar VP,Biswas S,Wuddie K,Stone S,Aschenake Z,Wilkins WL,Fam CM,Cox GN,Ghosh SP

14-6715-81 was used in Western Blotting to describe the use of a promising radiation countermeasure, BBT-059, and the results of a long term mouse study (up to 12 months) in the male CD2F1 strain using 60Co gamma irradiation (~0.6 Gy/min, 7.5-12.5 Gy).

Wed Apr 22 00:00:00 UTC 2020

Delayed effects of acute whole body lethal radiation exposure in mice pre-treated with BBT-059.

Scientific reports

Sharma NK,Holmes-Hampton GP,Kumar VP,Biswas S,Wuddie K,Stone S,Aschenake Z,Wilkins WL,Fam CM,Cox GN,Ghosh SP

Wed Apr 22 00:00:00 UTC 2020

An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7.

PloS one

Ghate NB,Chaudhuri D,Sarkar R,Sajem AL,Panja S,Rout J,Mandal N

14-6715 was used in Western Blotting to hypothesise that the antioxidant and anticancer potential of the PRME may reside in the phytoconstitutents present in it.

Tue Sep 30 00:00:00 UTC 2014

生物信息学

蛋白别名: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

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基因别名: CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; UV96; WAF1

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UniProt ID: (Human) P38936, (Mouse) P39689

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Entrez Gene ID: (Human) 1026, (Mouse) 12575, (Rat) 114851

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