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产品信息
PA5-32530
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from C-terminus of human p27
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 1% BSA
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2549996
产品详细信息
This antibody is predicted to react with canine, mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate or colon tissue.
靶标信息
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDN1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; P27 CDKN1B; p27(KIP1); p27Kip1
基因别名: CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1
UniProt ID: (Human) P46527
Entrez Gene ID: (Human) 1027
cyclin-dependent protein serine/threonine kinase inhibitor activity
transforming growth factor beta receptor, cytoplasmic mediator activity
protein binding
cysteine-type endopeptidase activator activity involved in apoptotic process
protein phosphatase binding
Hsp70 protein binding
protein complex binding
chaperone binding
kinase inhibitor
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
response to hypoxia
potassium ion transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
Notch signaling pathway
sensory perception of sound
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
positive regulation of cell death
negative regulation of cell growth
positive regulation of microtubule polymerization
response to estradiol
negative regulation of kinase activity
negative regulation of phosphorylation
response to drug
negative regulation of apoptotic process
response to amino acid
response to peptide hormone
positive regulation of protein catabolic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
response to cadmium ion
autophagic cell death
inner ear development
negative regulation of cellular component movement
negative regulation of epithelial cell proliferation involved in prostate gland development
cellular response to antibiotic
cellular response to lithium ion
cellular response to organic cyclic compound
mitotic cell cycle arrest
negative regulation of vascular smooth muscle cell proliferation
