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p47phox Antibody (PA5-27821) in ICC/IF
Immunofluorescence analysis of p47phox was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with p47phox Rabbit Polyclonal Antibody (Product # PA5-27821) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a d... View More
产品信息
PA5-27821
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 258 of Human NCF1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545297
产品详细信息
Recommended positive controls: Raji.
Predicted reactivity: Human (99%), Mouse (83%), Rat (80%), Pig (82%), Rabbit (86%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; SH3 and PX domain-containing protein 1A
基因别名: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
UniProt ID: (Human) P14598
Entrez Gene ID: (Human) 653361
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
protein targeting to membrane
superoxide metabolic process
cellular defense response
superoxide anion generation
positive regulation of catalytic activity
innate immune response
cell redox homeostasis
respiratory burst
vascular endothelial growth factor receptor signaling pathway
oxidation-reduction process
