Invitrogen

p73 Polyclonal Antibody

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2 篇参考文献

查看其他21个p73抗体

抗体检测数据 (4)
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p73 Antibody in Immunocytochemistry (ICC/IF)

p73 Antibody in Immunocytochemistry (ICC/IF)

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p73 Antibody (PA5-35368) in ICC/IF

Immunocytochemistry analysis of p73 in 293 cells. Samples were incubated in p73 polyclonal antibody (Product # PA5-35368) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

p73 Antibody in Immunocytochemistry (ICC/IF)

p73 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p73 Antibody in Western Blot (WB)

p73 Antibody in Flow Cytometry (Flow)

p73 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

p73 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of p73 in 293 cells. Samples were incubated in p73 polyclonal antibody (Product # PA5-35368) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

p73 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of p73 in formalin fixed and paraffin embedded mouse brain. Samples were incubated with p73 polyclonal antibody (Product # PA5-35368) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

p73 Antibody in Western Blot (WB) — Western blot analysis of p73 in K562 cell line and mouse brain tissue lysates. Samples were incubated with p73 polyclonal antibody (Product # PA5-35368). Lysates: 35 µg/lane. TP73 protein (arrow).

p73 Antibody in Flow Cytometry (Flow) — Flow cytometry of p73 in 293 cells (right histogram). Samples were incubated with p73 polyclonal antibody (Product # PA5-35368) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).

p73 Antibody in Immunocytochemistry (ICC/IF) — Figure 4 Deferasirox regulates p53 and p73 protein stability. ( A ) Immunofluorescence of p53 and p73 after DFX treatment. The green signal corresponds to p53 or p73 while the red propidium is used to detect nuclei (63x magnification). ( B ) p73 Immunohistochemistry on MDS bone marrow samples derived from 3 different patients at diagnosis (DX) and within one year of iron chelation treatment (ICT) (20x magnification). ( C ) Differentially expressed genes clusters enriched according to Gene Ontology terms and ordered by ascending adjusted p -value. ( D ) Schematic representation of the plausible role of iron chelation on mitochondrial activity and on p53 family stability (created in biorender.com). Abbreviations: NT, not treated; DFX, Deferasirox.

产品信息

PA5-35368

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

-

免疫细胞化学 (ICC/IF)

1:10-1:50

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:10-1:50

-

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 288-317 amino acids from the central region of human TP73

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2552678

靶标信息

P73 (TP73) participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Deferasirox-Dependent Iron Chelation Enhances Mitochondrial Dysfunction and Restores p53 Signaling by Stabilization of p53 Family Members in Leukemic Cells.

International journal of molecular sciences

Calabrese C,Panuzzo C,Stanga S,Andreani G,Ravera S,Maglione A,Pironi L,Petiti J,Shahzad Ali MS,Scaravaglio P,Napoli F,Fava C,De Gobbi M,Frassoni F,Saglio G,Bracco E,Pergolizzi B,Cilloni D

PA5-35368 was used in Immunocytochemistry-immunoflourescence to investigate the potential anti-leukemic effect of DFX, by functionally and molecularly analyzing its effects in three different leukemia cell lines, harboring or not p53 mutations, and in human primary cells derived from 15 MDS/AML patients.

Fri Oct 16 00:00:00 UTC 2020

Deferasirox-Dependent Iron Chelation Enhances Mitochondrial Dysfunction and Restores p53 Signaling by Stabilization of p53 Family Members in Leukemic Cells.

International journal of molecular sciences

Calabrese C,Panuzzo C,Stanga S,Andreani G,Ravera S,Maglione A,Pironi L,Petiti J,Shahzad Ali MS,Scaravaglio P,Napoli F,Fava C,De Gobbi M,Frassoni F,Saglio G,Bracco E,Pergolizzi B,Cilloni D

PA5-35368 was used in Immunocytochemistry-immunoflourescence to investigate the potential anti-leukemic effect of DFX, by functionally and molecularly analyzing its effects in three different leukemia cell lines, harboring or not p53 mutations, and in human primary cells derived from 15 MDS/AML patients.

Fri Oct 16 00:00:00 UTC 2020

Rapamycin enhances the sensitivity of ER‑positive breast cancer cells to tamoxifen by upregulating p73 expression.

Oncology reports

Zhu L,Li XX,Shi L,Wu J,Qian JY,Xia TS,Zhou WB,Sun X,Zhou XJ,Wei JF,Ding Q

PA5-35368 was used in Immunohistochemistry to maximize the benefits associated with tamoxifen therapy via drug combination strategies.

Tue Jan 01 00:00:00 UTC 2019

生物信息学

蛋白别名: OTTHUMP00000003479; OTTHUMP00000003480; p53-like transcription factor; p53-related protein; RP23-254N4.10; Tumor protein p73

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基因别名: deltaNp73; P73; TAp73; TP73; Trp73

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UniProt ID: (Human) O15350, (Mouse) Q9JJP2

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Entrez Gene ID: (Human) 7161, (Mouse) 22062

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding p53 binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein kinase binding identical protein binding transcription regulatory region DNA binding metal ion binding DNA binding double-stranded DNA binding sequence-specific DNA binding P53-like transcription factor

negative regulation of transcription from RNA polymerase II promoter activation of MAPK activity kidney development mismatch repair transcription from RNA polymerase II promoter cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator cell cycle arrest regulation of mitotic cell cycle intrinsic apoptotic signaling pathway in response to DNA damage response to X-ray response to organonitrogen compound response to gamma radiation regulation of gene expression viral process mitotic G1 DNA damage checkpoint cellular response to UV response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process negative regulation of JUN kinase activity negative regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of oligodendrocyte differentiation protein tetramerization negative regulation of cardiac muscle cell proliferation positive regulation of cell cycle arrest positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator release of cytochrome c from mitochondria transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process inflammatory response cell cycle post-embryonic development hippocampus development forebrain development cerebrospinal fluid secretion positive regulation of apoptotic process regulation of neuron apoptotic process positive regulation of cell size digestive tract morphogenesis neuron development positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of apoptotic signaling pathway