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14-3-3 beta Antibody (PA5-81126) in WB
Western Blot using 14-3-3 beta Polyclonal Antibody (Product # PA5-81126) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).
产品信息
PA5-81126
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human 14-3-3 beta/YWHAB protein (Met1-Asn246)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2788370
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human 14-3-3 beta/YWHAB.
靶标信息
14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 14-3-3 alpha; 14-3-3 protein beta/alpha; brain protein 14-3-3, beta isoform; epididymis secretory protein Li 1; KCIP 1; KCIP-1; KCIP1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; RP1-148E22.1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
基因别名: GW128; HEL-S-1; HS1; KCIP-1; YWHAA; YWHAB
UniProt ID: (Human) P31946
Entrez Gene ID: (Human) 7529
MAPK cascade
protein targeting
viral process
negative regulation of protein dephosphorylation
hippo signaling
positive regulation of catalytic activity
regulation of mRNA stability
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
cytoplasmic sequestering of protein
protein heterooligomerization
membrane organization
cell-cell adhesion
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
