Invitrogen

ADAMTS4 Polyclonal Antibody

ADAMTS4 Antibody in Immunocytochemistry (ICC/IF)

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ADAMTS4 Antibody (PA1-1749A) in ICC/IF

Immunofluorescence analysis of ADAMTS4 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ADAMTS4 Rabbit Polyclonal Antibody (Product # PA1-1749A) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

ADAMTS4 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-1749A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Bovine, Human, Mouse, Pig

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C V(394) M A H V D P E E P(403) of mouse ADAMTS4.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_1070004

产品详细信息

PA1-1749A detects ADAMTS4 from porcine, bovine, mouse and human samples. PA1-1749A is specific for ADAMTS4 and shows no cross-reactivity with other ADAMTS family members.

PA1-1749A has been successfully used in Western blot procedures. By Western blot, this antibody detects ADAMTS4 at ~68, ~53 and 30 kDa protein representing ADAMTS4 and its proteolytic fragments. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody.

PA1-1749A immunogen is a synthetic peptide corresponding to residues C V(394) M A H V D P E E P(403) of mouse ADAMTS4. PA1-1749A immunizing peptide (Cat. # PEP-234) is available for use in neutralization and control procedures. This polyclonal antibody has been referred to as: JSCVMA. PA1-1749A has been used in combination with PA1-1750 for detection of all ADAMTS4 processed forms.

靶标信息

This gene encodes a member of the ADAMTS protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Synergetic enrichment of aggrecan in nucleus pulposus cells by scAAV6-shRNA-mediated knockdown of aggrecanase-1 and aggrecanase-2.

Experimental biology and medicine (Maywood, N.J.)

Mern DS,Thomé C

Published figure using ADAMTS4 polyclonal antibody (Product # PA1-1749A) in Western Blot

Sat Jul 01 00:00:00 UTC 2023

Identification of Adamts4 as a novel adult cardiac injury biomarker with therapeutic implications in patients with cardiac injuries.

Scientific reports

Khanam R,Sengupta A,Mukhopadhyay D,Chakraborty S

Published figure using ADAMTS4 polyclonal antibody (Product # PA1-1749A) in Western Blot

Tue Jun 14 00:00:00 UTC 2022

Identification of Adamts4 as a novel adult cardiac injury biomarker with therapeutic implications in patients with cardiac injuries.

Scientific reports

Khanam R,Sengupta A,Mukhopadhyay D,Chakraborty S

Published figure using ADAMTS4 polyclonal antibody (Product # PA1-1749A) in Western Blot

Tue Jun 14 00:00:00 UTC 2022

ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries.

Nature communications

Kaufmann JO,Brangsch J,Kader A,Saatz J,Mangarova DB,Zacharias M,Kempf WE,Schwaar T,Ponader M,Adams LC,Möckel J,Botnar RM,Taupitz M,Mägdefessel L,Traub H,Hamm B,Weller MG,Makowski MR

Published figure using ADAMTS4 polyclonal antibody (Product # PA1-1749A) in Immunohistochemistry

Mon May 23 00:00:00 UTC 2022

Versican is differentially regulated in the adventitial and medial layers of human vein grafts.

PloS one

Kenagy RD,Kikuchi S,Evanko SP,Ruiter MS,Piola M,Longchamp A,Pesce M,Soncini M,Deglise S,Fiore GB,Haefliger JA,Schmidt TA,Majesky MW,Sobel M,Wight TN

PA1-1749A was used in Immunohistochemistry to examine the production and distribution of versican and hyaluronan in intact human vein rings cultured ex vivo, veins perfused ex vivo, and cultured venous adventitial and smooth muscle cells.

Wed Mar 06 00:00:00 UTC 2019

The anti-atherogenic cytokine interleukin-33 inhibits the expression of a disintegrin and metalloproteinase with thrombospondin motifs-1, -4 and -5 in human macrophages: Requirement of extracellular signal-regulated kinase, c-Jun N-terminal kinase and phosphoinositide 3-kinase signaling pathways.

The international journal of biochemistry & cell biology

Ashlin TG,Buckley ML,Salter RC,Johnson JL,Kwan AP,Ramji DP

PA1-1749A was used in immunohistochemistry - paraffin section to investigate the effect of IL-33 on the expression of ADAMTS-1, -4 and -5 in human macrophages and atherosclerotic lesions

Wed Jan 01 00:00:00 UTC 2014

ADAMTS-4 and biglycan are expressed at high levels and co-localize to podosomes during endothelial cell tubulogenesis in vitro.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Obika M,Vernon RB,Gooden MD,Braun KR,Chan CK,Wight TN

PA1-1749A was used in western blot to study the expression of ADAMTS-4 and biglycan during endothelial cell tubulogenesis using an in vitro model

Wed Jan 01 00:00:00 UTC 2014

Tumor necrosis factor-α induces ADAMTS-4 expression in human osteoarthritis chondrocytes.

Molecular medicine reports

Xue J,Wang J,Liu Q,Luo A

PA1-1749A was used in Western Blotting to demonstrate evidence of crosstalk between tumor necrosis factor-α and disintegrin and metalloproteinase with thrombospondin motifs 4 in relation to osteoarthritis cartilage degradation.

Sun Dec 01 00:00:00 UTC 2013

Regulated proteolytic processing of Reelin through interplay of tissue plasminogen activator (tPA), ADAMTS-4, ADAMTS-5, and their modulators.

PloS one

Krstic D,Rodriguez M,Knuesel I

PA1-1749A was used in immunohistochemistry and western blot to study the role of tPA, ADAMTS-4, ADAMTS-5, and endogenous proteinase inhibitors in modulating the proteolytic cleavage of Reelin

Tue Apr 09 00:00:00 UTC 2013

Regulated proteolytic processing of Reelin through interplay of tissue plasminogen activator (tPA), ADAMTS-4, ADAMTS-5, and their modulators.

PloS one

Krstic D,Rodriguez M,Knuesel I

PA1-1749A was used in immunohistochemistry and western blot to study the role of tPA, ADAMTS-4, ADAMTS-5, and endogenous proteinase inhibitors in modulating the proteolytic cleavage of Reelin

Tue Apr 09 00:00:00 UTC 2013

The expression of a disintegrin and metalloproteinase with thrombospondin motifs 4 in human macrophages is inhibited by the anti-atherogenic cytokine transforming growth factor-β and requires Smads, p38 mitogen-activated protein kinase and c-Jun.

The international journal of biochemistry & cell biology

Salter RC,Arnaoutakis K,Michael DR,Singh NN,Ashlin TG,Buckley ML,Kwan AP,Ramji DP

PA1-1749A was used in western blot to investigate the involvement of ADAMTS-4 in the anti-atherogenic effect of transforming growth factor

Sun May 01 00:00:00 UTC 2011

生物信息学

蛋白别名: a disintegrin and metalloproteinase; A disintegrin and metalloproteinase with thrombospondin motifs 4; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAM-TS4; ADAMs; ADAMTS-4; ADMP-1; Aggrecanase 1; Aggrecanase-1; metalloendopeptidases

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基因别名: 3830423K05; ADAM-TS4; ADAMTS-2; ADAMTS-4; ADAMTS4; ADMP-1; BOS_2624; KIAA0688; mKIAA0688; UNQ769/PRO1563

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UniProt ID: (Bovine) Q9TT93, (Human) O75173, (Mouse) Q8BNJ2

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Entrez Gene ID: (Bovine) 286806, (Human) 9507, (Mouse) 240913

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功能

metalloendopeptidase activity metallopeptidase activity zinc ion binding protease binding protein binding peptidase activity hydrolase activity metal ion binding

参与通路

proteolysis defense response to bacterium skeletal system development extracellular matrix disassembly