Invitrogen

AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience™

AGO2 Antibody in Immunocytochemistry (ICC/IF)

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AGO2 Antibody (14-6519-82) in ICC/IF

Immunofluorescent analysis of Ago2 (green) in U-2 OS cells (45% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37 C and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained with the Ago2 Monoclonal Antibody (Product # 14-6519-82) at a concentration of 1.25 µg/mL or Rat IgG2a Isotype Control at 4 C overnight in blocking buffer followed by a Goat anti-Rat IgG (H+L) Superclonal Sec... View More

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AGO2 Antibody — Western Blot analysis was performed using whole cell extracts of wild type (WT) 293T cells (Lane 1) and Ago2 knockout 293T cells generated by Crispr/Cas9 gene targeting (Lane 2) on a 4-12% Nupage Bis-Tris Gel (Product # NP0336BOX) for gel electrophoresis in MOPS SDS Running Buffer (Product # NP0001-02). Protein transfer to a nitrocellulose membrane was achieved using the iBlot 2 system. The membrane was probed with Ago2 Monoclonal Antibody (clone AGO2 11A9, 4 µg/mL) and detected by chemiluminescence using Goat-anti Rat IgG Secondary Antibody, HRP (Product # 31470) at 1:10,000 dilution. This Ago2 Monoclonal Antibody detects a 93 kDa band in WT 293T cells but not in Ago2 knockout lysate using Super Signal West Pico chemiluminescence substrate. Protein size was determined using Page Ruler Pre-stained Protein Standard (Product # 26616). {KO}

产品信息

14-6519-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

4.0 µg/mL

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免疫细胞化学 (ICC/IF)

1.25 µg/mL

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

AGO2 11A9

抗原

Peptide of human N-terminus

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2784637

产品详细信息

Description: This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), which has been described as a transcriptional regulator by means of RNA interference and endonucleolytic cleavage. The epitope recognized by AGO2 11A9 detects reduced Ago2 by Western Blot and stains Ago2 in the cytoplasm and nucleus in Immunocytochemistry. Ago2 monoclonal antibody (AGO2 11A9) has been validated for the following applications: Western Blot and Immunocytochemistry; AGO2 11A9 does not cross react with murine Ago2.

Applications Reported: This AGO2 11A9 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.

Applications Tested: This AGO2 11A9 antibody has been tested by western blot using lysates from different human cancer cell lines and by immunocytochemistry/immunofluorescence using fixed human U-2 OS cells. This may be used at less than or equal to 4 µg/mL for western blot and at less than or equal to 1.25 µg/mL for immunocytochemistry/immunofluorescence.

靶标信息

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or ″Slicer″ activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Silencing of Ago-2 Interacting Protein SERBP1 Relieves KCC2 Repression by miR-92 in Neurons.

Cells

Barbato C,Frisone P,Braccini L,D'Aguanno S,Pieroni L,Ciotti MT,Catalanotto C,Cogoni C,Ruberti F

Published figure using AGO2 monoclonal antibody (Product # 14-6519-82) in Western Blot

Sun Mar 20 00:00:00 UTC 2022

No evidence for Ago2 translocation from the host erythrocyte into the Plasmodium parasite.

Wellcome open research

Hentzschel F,Obrova K,Marti M

14-6519-82 was used in Immunocytochemistry to show that previously observed Ago2 localisation in the parasite by fluorescence microscopy is the result of antibody cross reactivity.

Fri Jan 29 00:00:00 UTC 2021

No evidence for Ago2 translocation from the host erythrocyte into the Plasmodium parasite.

Wellcome open research

Hentzschel F,Obrova K,Marti M

14-6519-82 was used in Immunocytochemistry to show that previously observed Ago2 localisation in the parasite by fluorescence microscopy is the result of antibody cross reactivity.

Fri Jan 29 00:00:00 UTC 2021

生物信息学

蛋白别名: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; CTA-204B4.6; eIF-2C 1; eIF-2C 2; eIF2C; eIF2C 1; eIF2C 2; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 2; hAgo1; hAgo2; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer

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基因别名: AGO2; EIF2C2; Q10

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UniProt ID: (Human) Q9UKV8

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Entrez Gene ID: (Human) 27161

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功能