Invitrogen

AHNAK Monoclonal Antibody (EM-09)

AHNAK Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

AHNAK Antibody (MA1-10050) in ICC/IF

Immunofluorescence analysis of AHNAK was performed using 70% confluent log phase HaCaT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AHNAK Monoclonal Antibody (EM-09) (Product # MA1-10050, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:... View More

AHNAK Antibody in Immunohistochemistry (Frozen) (IHC (F))

AHNAK Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

AHNAK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of AHNAK was observed in U-87 MG, PANC-1, 769-P, NIH: OVCAR-3, HaCaT which are high expressing cell lines and was not observed in Jurkat, A-673, HEL 92.1.7 which are low expressing cell lines, using AHNAK Monoclonal Antibody (EM-09) (Product # MA1-10050) in Western Blot. {RE}

产品信息

MA1-10050

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

EM-09

抗原

Bacterially expressed fragment of N-terminal domain of human AHNAK1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_11156613

产品详细信息

This antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Immunocytochemistry: permeabilization is required.

靶标信息

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。