Invitrogen

APOE Recombinant Rabbit Monoclonal Antibody (16H22L18)

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APOE Antibody (701241) in WB

Western blot was performed using Anti-APOE Recombinant Rabbit Monoclonal Antibody (16H22L18) (Product # 701241) and a 34 kDa band corresponding to APOE was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 2), Caco-2 (Lane 3), Caco-2 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 4), A-431 (Lane 5), A-431 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 6), Daudi (Lane... View More

APOE Antibody in Immunocytochemistry (ICC/IF)

APOE Antibody in Immunohistochemistry (Frozen), Proximity Ligation Assay (PLA) (IHC (F), PLA)

APOE Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

APOE Antibody in Immunohistochemistry, Proximity Ligation Assay (PLA) (IHC, PLA)

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APOE Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of APOE was observed in Hep G2, CaCo-2 as compared to A-431, Daudi using Anti-APOE Recombinant Rabbit Monoclonal Antibody (16H22L18) (Product # 701241) in Western Blot. {RE}

产品信息

701241

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1-3 µg/mL

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免疫沉淀 (IP)

- -

in situ PLA (PLA)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

16H22L18

抗原

Peptide corresponding to amino acids 240-251 of human ApoE

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532438

产品详细信息

This antibody is predicted to react with non-human primate and mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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701241 was used in Immunohistochemistry to demonstrate that C/EBPβ/AEP signaling couples ATH to AD via mediating vascular pathology.

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701241 was used in Immunohistochemistry (Frozen) to demonstrate that the interactions of endothelial CD31 with monomeric C-reactive protein (mCRP) versus ApoE were linked with shortened neurovasculature for Alzheimer's disease (AD) pathology and cognition.

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Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

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Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

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Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

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Mon Nov 01 00:00:00 UTC 2021

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

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701241 was used in Western Blotting to find that C/EBPβ selectively promotes more ApoE4 expression versus ApoE3 in human neurons, correlating with higher activation of C/EBPβ in human AD brains with ApoE4/4 compared to ApoE3/3.

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