Invitrogen

Adipolean variant Polyclonal Antibody, Biotin, PeproTech®

Adipolean variant Antibody in Western Blot (WB)

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Adipolean variant Antibody (500-P205BT-25UG) in WB

Western Blot: To detect Human Adipolean Variant by Western Blot analysis Adipolean variant Polyclonal Antibody, Biotin (Product # 500-P205BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Adipolean variant Antibody in ELISA (ELISA)

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产品信息

500-P205BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human gAcrp30/Adipolean Variant

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929808

产品详细信息

AA Sequence of recombinant protein: PGAEGPRGFP GIQGRKGEPG EGAYVYRSAF SVGLETYVTI PNMPIRFTKI FYNQQNHYDG STGKFHCNIP GLYYFAYHIT VYMKDVKVSL FKKDKAMLFT YDQYQENNVD QASGSVLLHL EVGDQVWLQV YGEGERNGLY ADNDNDSTFT GFLLYHDTN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human gAcrp30/Adipolean Variant. Anti-Human gAcrp30/Adipolean Variant specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hAdipolean Variant by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean Variant (500-P205) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean Variant.

Western Blot: To detect Human Adipolean Variant by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P205BT-1MG will be provided as 2 x 500 µg

靶标信息

The gAcrp30 variant is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, Acrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. This naturally occurring variant of human gAcrp30/Adipolean is an 18.1 kDa protein, containing 14 extra amino acids extra at the N-terminus of human gAcrp30/Adipolean.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 30 kDa adipocyte complement-related protein; ACRP30; Adipocyte complement-related 30 kDa protein; Adipocyte, C1q and collagen domain-containing protein; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; apM-1; gAcrp30; Gelatin-binding protein; gelatin-binding protein 28

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基因别名: ACDC; ACRP30; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; GBP28

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UniProt ID: (Human) Q15848

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Entrez Gene ID: (Human) 9370

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功能

receptor binding cytokine activity hormone activity protein binding sialic acid binding identical protein binding protein homodimerization activity

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