Invitrogen

Axl Monoclonal Antibody (DS7HAXL), APC, eBioscience™

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Axl Antibody (17-1087-42) in Flow

Normal human peripheral blood cells were stained with HLA-DR Monoclonal Antibody, FITC (Product # 11-9956-42), CD1c Monoclonal Antibody, PE (Product # 12-0015-42), CD11c Monoclonal Antibody, eFluor 450 (Product # 49-0116-42), and Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or Axl Monoclonal Antibody, APC (right). CD11c+/CD14- cells were used for analysis.

Product Image Gallery

Axl Antibody — Antibody clone (DS7HAXL) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in Axl KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using Axl antibody (DS7HAXL). Yellow histogram represents staining with the isotype control. {KO}

产品信息

17-1087-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

DS7HAXL

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2723957

产品详细信息

This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis.

Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

靶标信息

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Oral Epithelial Cells Expressing Low or Undetectable Levels of Human Angiotensin-Converting Enzyme 2 Are Susceptible to SARS-CoV-2 Virus Infection In Vitro.

Pathogens (Basel, Switzerland)

Ebraham L,Xu C,Wang A,Hernandez C,Siclari N,Rajah D,Walter L,Marras SAE,Tyagi S,Fine DH,Daep CA,Chang TL

Published figure using Axl monoclonal antibody (Product # 17-1087-42) in Flow Cytometry

Mon Jun 19 00:00:00 UTC 2023

Single-cell profiling of alveolar rhabdomyosarcoma reveals RAS pathway inhibitors as cell-fate hijackers with therapeutic relevance.

Science advances

Danielli SG,Porpiglia E,De Micheli AJ,Navarro N,Zellinger MJ,Bechtold I,Kisele S,Volken L,Marques JG,Kasper S,Bode PK,Henssen AG,Gürgen D,Delattre O,Surdez D,Roma J,Bühlmann P,Blau HM,Wachtel M,Schäfer BW