Invitrogen

BAF53A/BAF53B Polyclonal Antibody

BAF53A/BAF53B Antibody in Immunocytochemistry (ICC/IF)

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BAF53A/BAF53B Antibody (PA5-18272) in ICC/IF

Immunocytochemistry analysis of BAF53A/BAF53B using BAF53A/BAF53B Polyclonal Antibody (Product # PA5-18272) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmu... View More

BAF53A/BAF53B Antibody in Western Blot (WB)

BAF53A/BAF53B Antibody in Flow Cytometry (Flow)

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产品信息

PA5-18272

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

10 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (YEEGGKQCVERKCP) corresponding to the C-terminus amino acids of ACTL6A / ACTL6B

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10985710

产品详细信息

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

靶标信息

The protein encoded by this gene is a member of a family of actin-related proteins which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 53 kDa BRG1-associated factor A; 53 kDa BRG1-associated factor B; actin-like 6; actin-like 6A; Actin-like protein 6A; Actin-like protein 6B; actin-like protein 6B {ECO:0000250|UniProtKB:O94805}; actin-related protein 4; Actin-related protein Baf53a; Actin-related protein Baf53b; actl6b {ECO:0000250|UniProtKB:O94805}; ArpN-alpha; ArpNa; ArpNalpha; ArpNbeta; BAF complex 53 kDa subunit; BAF53; BAF53A; BAF53B; BRG1-associated factor 53A; BRG1-associated factor 53B; BRG1/brm-associated factor 53A; hArpN alpha; hArpN beta; INO80 complex subunit K; MGC5382 Arp4

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基因别名: 2810432C06Rik; ACTL6; ACTL6A; ACTL6B; AI851094; ARP4; ARPN-BETA; ArpNa; arpNalpha; BAF53; BAF53A; BAF53B; C79802; INO80K; RGD1307747