Invitrogen

BCL7A Polyclonal Antibody

BCL7A Antibody in Immunocytochemistry (ICC/IF)

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BCL7A Antibody (PA5-17933) in ICC/IF

Immunocytochemistry analysis of BCL7A using BCL7A Polyclonal Antibody (Product # PA5-17933) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

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产品信息

PA5-17933

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (MKLEASQQNSEEM) corresponding to the C-terminus amino acids of BCL7A (aa 198-210).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10977865

产品详细信息

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.

靶标信息

This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。