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BMAL1 Antibody (MA5-32750) in ICC/IF
Immunofluorescence analysis of Arntl was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with BMAL1 Recombinant Rabbit Monoclonal Antibody (JM17-34) (Product # MA5-32750) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More
产品信息
MA5-32750
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JM17-34
抗原
Synthetic peptide within Human BMAL1 aa 550-599
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2810027
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARNT-like protein 1, brain and muscle; Arnt3; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic helix-loop-helix ARNT-like protein 1; basic-helix-loop-helix-PAS orphan MOP3; Basic-helix-loop-helix-PAS protein MOP3; bHLH-PAS protein JAP3; bHLHe5; Brain and muscle ARNT-like 1; CG8727 PA; Class E basic helix-loop-helix protein 5; cycle; Member of PAS protein 3; member of PAS superfamily 3; MGC47515; PAS domain-containing protein 3; testis tissue sperm-binding protein Li 50e; Tic
基因别名: Arnt3; ARNTL; BHLHE5; BMAL1; BMAL1b; bmal1b'; BMAL1c; JAP3; MOP3; PASD3; TIC
UniProt ID: (Human) O00327, (Rat) Q9EPW1, (Mouse) Q9WTL8
Entrez Gene ID: (Human) 406, (Rat) 29657, (Mouse) 11865
transcription regulatory region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription factor binding
DNA binding
protein binding
aryl hydrocarbon receptor binding
bHLH transcription factor binding
sequence-specific DNA binding
protein heterodimerization activity
Hsp90 protein binding
repressing transcription factor binding
E-box binding
transcription factor activity, sequence-specific DNA binding
signal transducer activity
protein dimerization activity
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
basic helix-loop-helix transcription factor
protein import into nucleus, translocation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
spermatogenesis
circadian rhythm
negative regulation of TOR signaling
circadian regulation of gene expression
regulation of protein catabolic process
regulation of hair cycle
positive regulation of circadian rhythm
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of neurogenesis
regulation of insulin secretion
regulation of cell cycle
response to redox state
maternal process involved in parturition
positive regulation of canonical Wnt signaling pathway
oxidative stress-induced premature senescence
regulation of type B pancreatic cell development
negative regulation of glucocorticoid receptor signaling pathway
regulation of cellular senescence
positive regulation of skeletal muscle cell differentiation
transcription, DNA-templated
signal transduction
regulation of circadian rhythm
rhythmic process
