Invitrogen

BMPR2 Monoclonal Antibody (3F6F8)

BMPR2 Antibody in Immunocytochemistry (ICC/IF)

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BMPR2 Antibody (MA5-15827) in ICC/IF

Immunofluorescent analysis of BMPR2 (green) in HepG2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20c, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a BMPR2 mouse monoclonal antibody (Product # MA5-15827) at a concentration of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32723) at a dilution of 1... View More

BMPR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BMPR2 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

BMPR2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

BMPR2 Antibody — Fig. 3 Antibody 3F6 has no effect on BMP-responsiveness in BMPR2 knock-down HEK293T cells. a Expression levels of endogenous BMPR2 in scramble control HEK293T cells (Control) and HEK293T cells carrying anti-BMPR2 shRNA (BMPR2-KD) compared to HPRT1 loading control. Data are expressed as normalized to scramble control (Control) using the 2^-ΔΔCt method. n= 6 per condition. Asterisk indicates p< 0.05 by unpaired t test. b, c Expression of endogenous BMPR2 in scramble control HEK293T cells (Control) and BMPR2-KD HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated in b. Representative immunoblot is shown in b and results from three independent runs are quantified in c (data are expressed as mean ± SEM ratio of BMPR2: β-actin normalized to scramble control (Relative Expression)). Asterisk indicates p< 0.05 by paired t test. d, e BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in BMPR2-KD HEK293T cells. No effect on BMP2-responsiveness of BMPR2-KD HEK293T cells was observed with pre-treatment using control ascites (Ctrl Asc.) or 3F6. Approximate molecular weights are indicated in d. Results are quantified in E and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone (Relative pS1:S1). n= 4 per condition {KD}

产品信息

MA5-15827

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

2-4 ug/mL

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免疫细胞化学 (ICC/IF)

1:100-1:200

流式细胞分析 (Flow)

1:100-1:200

酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Bovine, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3F6F8

抗原

Purified recombinant fragment of human BMPR2 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_11156844

产品详细信息

MA5-15827 targets BMPR2 in FACS, ICC/IF, IHC (P), and WB applications and shows reactivity with Human, mouse, Non-human primate, and rat samples.

靶标信息

BMPR2 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs that are involved in endochondral bone formation and embryogenesis. The loss of interaction and lack of phosphorylation of TCTEL1 by BMPR2 may contribute to the pathogenesis of primary pulmonary hypertension (PPH) and BMPR2 also plays an essential role in human T-cell differentiation. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in BMPR2 have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Exacerbated inflammatory signaling underlies aberrant response to BMP9 in pulmonary arterial hypertension lung endothelial cells.

Angiogenesis

Szulcek R,Sanchez-Duffhues G,Rol N,Pan X,Tsonaka R,Dickhoff C,Yung LM,Manz XD,Kurakula K,Kiełbasa SM,Mei H,Timens W,Yu PB,Bogaard HJ,Goumans MJ

MA5-15827 was used in Western Blotting to show that the BMP9-induced aberrant EndMT in PAH pulmonary ECs is dependent on exacerbated pro-inflammatory signaling mediated through IL6.

Sun Nov 01 00:00:00 UTC 2020

Exacerbated inflammatory signaling underlies aberrant response to BMP9 in pulmonary arterial hypertension lung endothelial cells.

Angiogenesis

Szulcek R,Sanchez-Duffhues G,Rol N,Pan X,Tsonaka R,Dickhoff C,Yung LM,Manz XD,Kurakula K,Kiełbasa SM,Mei H,Timens W,Yu PB,Bogaard HJ,Goumans MJ

MA5-15827 was used in Western Blotting to show that the BMP9-induced aberrant EndMT in PAH pulmonary ECs is dependent on exacerbated pro-inflammatory signaling mediated through IL6.

Sun Nov 01 00:00:00 UTC 2020

The BMP Receptor 2 in Pulmonary Arterial Hypertension: When and Where the Animal Model Matches the Patient.

Cells

Happé C,Kurakula K,Sun XQ,da Silva Goncalves Bos D,Rol N,Guignabert C,Tu L,Schalij I,Wiesmeijer KC,Tura-Ceide O,Vonk Noordegraaf A,de Man FS,Bogaard HJ,Goumans MJ

MA5-15827 was used in Western Blotting to analyse the BMPR2 expression and activity in the lungs of rats with experimentally induced PAH and compared this to the BMPR2 expression and activity in the lungs of PAH patients.

Mon Jun 08 00:00:00 UTC 2020

The BMP Receptor 2 in Pulmonary Arterial Hypertension: When and Where the Animal Model Matches the Patient.

Cells

Happé C,Kurakula K,Sun XQ,da Silva Goncalves Bos D,Rol N,Guignabert C,Tu L,Schalij I,Wiesmeijer KC,Tura-Ceide O,Vonk Noordegraaf A,de Man FS,Bogaard HJ,Goumans MJ

Mon Jun 08 00:00:00 UTC 2020

The BMP Receptor 2 in Pulmonary Arterial Hypertension: When and Where the Animal Model Matches the Patient.

Cells

Happé C,Kurakula K,Sun XQ,da Silva Goncalves Bos D,Rol N,Guignabert C,Tu L,Schalij I,Wiesmeijer KC,Tura-Ceide O,Vonk Noordegraaf A,de Man FS,Bogaard HJ,Goumans MJ

Mon Jun 08 00:00:00 UTC 2020

The BMP Receptor 2 in Pulmonary Arterial Hypertension: When and Where the Animal Model Matches the Patient.

Cells

Happé C,Kurakula K,Sun XQ,da Silva Goncalves Bos D,Rol N,Guignabert C,Tu L,Schalij I,Wiesmeijer KC,Tura-Ceide O,Vonk Noordegraaf A,de Man FS,Bogaard HJ,Goumans MJ

Mon Jun 08 00:00:00 UTC 2020

BMPR2 promotes fatty acid oxidation and protects white adipocytes from cell death in mice.

Communications biology

Qian S,Pan J,Su Y,Tang Y,Wang Y,Zou Y,Zhao Y,Ma H,Zhang Y,Liu Y,Guo L,Tang QQ

Published figure using BMPR2 monoclonal antibody (Product # MA5-15827) in Western Blot

Wed Apr 29 00:00:00 UTC 2020

BMPR2 promotes fatty acid oxidation and protects white adipocytes from cell death in mice.

Communications biology

Qian S,Pan J,Su Y,Tang Y,Wang Y,Zou Y,Zhao Y,Ma H,Zhang Y,Liu Y,Guo L,Tang QQ

Published figure using BMPR2 monoclonal antibody (Product # MA5-15827) in Western Blot

Wed Apr 29 00:00:00 UTC 2020

Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody.

BMC research notes

Gorrell RE,Totten MH,Schoerning LJ,Newby JB,Geyman LJ,Lawless WG,Hum JM,Lowery JW

MA5-15827 was used in Immunoprecipitation to identify and validate a bone morphogenic protein type 2receptor neutralising antibody.

Tue Jun 11 00:00:00 UTC 2019

Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody.

BMC research notes

Gorrell RE,Totten MH,Schoerning LJ,Newby JB,Geyman LJ,Lawless WG,Hum JM,Lowery JW

MA5-15827 was used in Immunoprecipitation to identify and validate a bone morphogenic protein type 2receptor neutralising antibody.

Tue Jun 11 00:00:00 UTC 2019

The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency.

Journal of virology

Workman A,Zhu L,Keel BN,Smith TPL,Jones C

Published figure using BMPR2 monoclonal antibody (Product # MA5-15827) in Immunofluorescence

Sun Apr 01 00:00:00 UTC 2018

Mitomycin-Induced Pulmonary Veno-Occlusive Disease: Evidence From Human Disease and Animal Models.

Circulation

Perros F,Günther S,Ranchoux B,Godinas L,Antigny F,Chaumais MC,Dorfmüller P,Hautefort A,Raymond N,Savale L,Jaïs X,Girerd B,Cottin V,Sitbon O,Simonneau G,Humbert M,Montani D

Published figure using BMPR2 monoclonal antibody (Product # MA5-15827) in Western Blot

Tue Sep 01 00:00:00 UTC 2015

Mitomycin-Induced Pulmonary Veno-Occlusive Disease: Evidence From Human Disease and Animal Models.

Circulation

Perros F,Günther S,Ranchoux B,Godinas L,Antigny F,Chaumais MC,Dorfmüller P,Hautefort A,Raymond N,Savale L,Jaïs X,Girerd B,Cottin V,Sitbon O,Simonneau G,Humbert M,Montani D

Published figure using BMPR2 monoclonal antibody (Product # MA5-15827) in Western Blot

Tue Sep 01 00:00:00 UTC 2015

Mitomycin-Induced Pulmonary Veno-Occlusive Disease: Evidence From Human Disease and Animal Models.

Circulation

Perros F,Günther S,Ranchoux B,Godinas L,Antigny F,Chaumais MC,Dorfmüller P,Hautefort A,Raymond N,Savale L,Jaïs X,Girerd B,Cottin V,Sitbon O,Simonneau G,Humbert M,Montani D

Published figure using BMPR2 monoclonal antibody (Product # MA5-15827) in Western Blot

Tue Sep 01 00:00:00 UTC 2015

Endothelial-to-mesenchymal transition in pulmonary hypertension.

Circulation

Ranchoux B,Antigny F,Rucker-Martin C,Hautefort A,Péchoux C,Bogaard HJ,Dorfmüller P,Remy S,Lecerf F,Planté S,Chat S,Fadel E,Houssaini A,Anegon I,Adnot S,Simonneau G,Humbert M,Cohen-Kaminsky S,Perros F

MA5-15827 was used in Western Blotting to demonstrate that rapamycin partially reverses the protein expression patterns of endothelial-to-mesenchymal transition (EndoMT), improves experimental pulmonary arterial hypertension, and decreases the migration of human pulmonary artery endothelial cells.

Tue Mar 17 00:00:00 UTC 2015

Endothelial-to-mesenchymal transition in pulmonary hypertension.

Circulation

Tue Mar 17 00:00:00 UTC 2015

生物信息学

蛋白别名: BMP type II receptor; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; Bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); bone morphogenic protein receptor, type II (serine/threonine kinase); BRK-3; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4

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基因别名: 2610024H22Rik; AL117858; AW546137; BB189135; BMP-2; BMPR-2; BMPR-II; BMPR2; BMPR3; BMPRII; BMR2; BRK-3; Gm20272; POVD1; PPH1; T-ALK

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UniProt ID: (Human) Q13873, (Mouse) O35607

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Entrez Gene ID: (Human) 659, (Rat) 140590, (Mouse) 12168

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功能