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Brachyury Antibody (PA5-46984) in ICC/IF
Immunocytochemistry analysis of Brachyury in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm. Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was locali... View More
产品信息
PA5-46984
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant human Brachyury Ser2-Glu202
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610378
产品详细信息
In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Brachyury protein; brachyury-like 2; brachyury-like 3; low ratio; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T
基因别名: Bra; cou; D17Mit170; Low; Lr; me75; SAVA; T; T1; TBXT; TFT; Tl2; Tl3
UniProt ID: (Human) O15178, (Mouse) P20293
Entrez Gene ID: (Human) 6862, (Mouse) 20997
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
protein binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
somitogenesis
neural plate morphogenesis
neural tube closure
heart morphogenesis
positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation
transcription, DNA-templated
signal transduction
penetration of zona pellucida
mesoderm development
mesoderm migration involved in gastrulation
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
notochord formation
signal transduction involved in regulation of gene expression
BMP signaling pathway
post-anal tail morphogenesis
embryonic skeletal system development
canonical Wnt signaling pathway
bone morphogenesis
SMAD protein signal transduction
determination of heart left/right asymmetry
cellular response to retinoic acid
primitive streak formation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
anatomical structure morphogenesis
anterior/posterior pattern specification
reproductive process
notochord development
positive regulation of transcription from RNA polymerase II promoter
cardiac muscle cell differentiation
