Invitrogen

Brachyury Monoclonal Antibody (1H9A2)

查看其他33个Brachyury抗体

抗体检测数据 (6)

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 6

Brachyury Antibody (MA5-17185) in IHC (P)

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Brachyury protein T monoclonal antibody (Product # MA5-17185) followed with DAB staining.

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Brachyury Antibody in Western Blot (WB)

Brachyury Antibody in Western Blot (WB)

Brachyury Antibody in Western Blot (WB)

Brachyury Antibody in Flow Cytometry (Flow)

Product Image Gallery

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Brachyury protein T monoclonal antibody (Product # MA5-17185) followed with DAB staining.

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Brachyury protein T monoclonal antibody (Product # MA5-17185) followed with DAB staining.

Brachyury Antibody in Western Blot (WB) — Western blot analysis of Brachyury protein T using Brachyury protein T monoclonal antibody (Product # MA5-17185) in Raji (1), and Jurkat (2) cell lysate.

Brachyury Antibody in Western Blot (WB) — Western blot analysis of Brachyury protein T using Brachyury protein T monoclonal antibody (Product # MA5-17185) in HEK293 (1) and T (AA: 257-309) human IgG Fc transfected HEK293 (2) cell lysate.

Brachyury Antibody in Western Blot (WB) — Western blot analysis of Brachyury protein T using a Brachyury protein T monoclonal antibody (Product # MA5-17185) against a human T recombinant protein.

Brachyury Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HeLa cells using Brachyury protein T monoclonal antibody (Product # MA5-17185) (green) and negative control (red).

产品信息

MA5-17185

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

-

流式细胞分析 (Flow)

1:200-1:400

-

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1H9A2

抗原

Purified recombinant fragment of human T (amino acids: 257-309 ) expressed in E. Coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538656

产品详细信息

MA5-17185 targets Brachyury protein T in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples.

The MA5-17185 immunogen is purified recombinant fragment of human T (amino acids: 257-309 ) expressed in E. Coli.

MA5-17185 detects Brachyury protein T which has a predicted molecular weight of approximately 47.4kDa.

靶标信息

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Brachyury protein; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

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基因别名: SAVA; T; TBXT; TFT

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UniProt ID: (Human) O15178

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Entrez Gene ID: (Human) 6862

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Rel homology transcription factor

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation