Rockland

BrdU Monoclonal Antibody (29G6.E8)

产品信息

200-301-H50

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:5,000

免疫组化 (IHC)

1:100-1:500

流式细胞分析 (Flow)

1:50-1:100

酶联免疫吸附实验 (ELISA)

1:2,000-1:10,000

产品规格

种属反应

Chemical

宿主/亚型

Mouse / IgG1, lambda

分类

Monoclonal

类型

Antibody

克隆号

29G6.E8

抗原

Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.

靶标信息

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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