Invitrogen

C1QBP Polyclonal Antibody

C1QBP Antibody in Immunocytochemistry (ICC/IF)

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C1QBP Antibody (PA5-117816) in ICC/IF

Immunofluorescence staining of His-mC1QBP in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with C1QBP Polyclonal Antibody (Product # PA5-117816, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

C1QBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C1QBP Antibody in Immunoprecipitation (IP)

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产品信息

PA5-117816

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

1-5 µL/mg of lysate

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Mouse C1QBP/HABP1 Protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2902425

靶标信息

Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) is involved in DNA replication, repair, and recombination. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。