Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (4E2), eBioscience™

产品信息

14-7091-81

应用

建议稀释比

已发表文章

酶联免疫吸附实验 (ELISA)

1-4 µg/mL

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中和实验 (Neu)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

4E2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468428

产品详细信息

Description: The 4E2/MCP antibody reacts with mouse monocyte chemoattractant protein-1 (MCP-1).

Applications Reported: This 4E2 antibody has been reported for use in ELISA.

Applications Tested: The 4E2 antibody has been tested as the capture antibody in a sandwich ELISA in combination with the Biotin 2H5 (Product # 13-7096-85) antibody for detection of mouse CCL2 (MCP-1, MCP1, MCAF). A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The liver X receptor agonist GW3965 improves recovery from mild repetitive traumatic brain injury in mice partly through apolipoprotein E.

PloS one

Namjoshi DR,Martin G,Donkin J,Wilkinson A,Stukas S,Fan J,Carr M,Tabarestani S,Wuerth K,Hancock RE,Wellington CL

14-7091 was used in an ELISA assay to investigate the therapeutic use of Liver X receptor for treatment of TBI.

Tue Jul 09 00:00:00 UTC 2013

CCR2 defines a distinct population of NK cells and mediates their migration during influenza virus infection in mice.

PloS one

van Helden MJ,Zaiss DM,Sijts AJ

14-7091 was used in an ELISA assay to determine the role of CCR2 in NK cell migration during intranasal influenza virus infection, showing that it defines a distinct population of NK cells and mediates their migration.

Mon Jun 10 00:00:00 UTC 2013

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE

14-7091 was used in an ELISA assay to study the importance of the chemokine induction activity of host defence peptides in protection against bacterial infections.

Mon Mar 01 00:00:00 UTC 2010

Prophylaxis of lipopolysaccharide-induced shock by alpha-galactosylceramide.

Journal of leukocyte biology

Sireci G,La Manna MP,Di Liberto D,Lo Dico M,Taniguchi M,Dieli F,Salerno A

14-7091-81 was used in Neutralization to demonstrate that KRN7000, injected from 6 to 12 days before the first administration of lipopolysaccharide, prevents endotoxin shock by inhibiting interferon-gamma, TNF-alpha, and MCP-1 release.

Fri Aug 01 00:00:00 UTC 2008

生物信息学

蛋白别名: C-C motif chemokine 2; H-MCP-1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; Small-inducible cytokine A2

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基因别名: AI323594; Ccl2; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF

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UniProt ID: (Mouse) P10148

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Entrez Gene ID: (Mouse) 20296

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功能

G-protein coupled receptor binding cytokine activity protein binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule

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