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CD104 (Integrin beta 4) Antibody (13-1049-82) in Flow
Staining of the A549 cell line with 0.125 µg of Rat IgG2b K Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.125 µg of Anti-Human CD104 (Integrin beta 4) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
产品信息
13-1049-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
439-9B
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1210583
产品详细信息
Description: The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta 4. CD104 is a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine.
Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.
Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of EDTA treated A549 cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD antigen CD104; CD104; CD104 antigen; GP150; Integrin beta-4
基因别名: CD104; GP150; ITGB4
UniProt ID: (Human) P16144
Entrez Gene ID: (Human) 3691
autophagy
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
response to wounding
extracellular matrix organization
hemidesmosome assembly
nail development
skin development
mesodermal cell differentiation
digestive tract development
cell motility
renal system development
amelogenesis
