Invitrogen

CD127 Monoclonal Antibody (eBioRDR5), APC-eFluor™ 780, eBioscience™

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CD127 Antibody (47-1278-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 kappa Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD127 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

47-1278-42

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看24篇已发表文章 24篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioRDR5

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1548674

产品详细信息

Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.

Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (Product # 14-1079-80).

Applications Reported: This eBioRDR5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioRDR5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

47-1278-42 was used in Flow cytometry/Cell sorting to demonstrate a critical role for antigen-presenting ILC3s in orchestrating immune tolerance in the airway by restricting pro-inflammatory T cell responses to both allergens and microbes.

Tue Nov 23 00:00:00 UTC 2021

Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

Yang P,Tian H,Zou YR,Chambon P,Ichinose H,Honig G,Diamond B,Kim SJ

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Mon Jul 05 00:00:00 UTC 2021

A controlled human Schistosoma mansoni infection model to advance novel drugs, vaccines and diagnostics.

Nature medicine

Langenberg MCC,Hoogerwerf MA,Koopman JPR,Janse JJ,Kos-van Oosterhoud J,Feijt C,Jochems SP,de Dood CJ,van Schuijlenburg R,Ozir-Fazalalikhan A,Manurung MD,Sartono E,van der Beek MT,Winkel BMF,Verbeek-Menken PH,Stam KA,van Leeuwen FWB,Meij P,van Diepen A,van Lieshout L,van Dam GJ,Corstjens PLAM,Hokke CH,Yazdanbakhsh M,Visser LG,Roestenberg M

47-1278 was used in Flow cytometry/Cell sorting to performed a dose-escalating clinical safety trial in 17 volunteers with male Schistosoma mansoni cercariae, which do not produce eggs (clinicaltrials.gov NCT02755324), at the Leiden University Medical Center, the Netherlands.

Sun Mar 01 00:00:00 UTC 2020

A probiotic modulates the microbiome and immunity in multiple sclerosis.

Annals of neurology

Tankou SK,Regev K,Healy BC,Tjon E,Laghi L,Cox LM,Kivisäkk P,Pierre IV,Hrishikesh L,Gandhi R,Cook S,Glanz B,Stankiewicz J,Weiner HL

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Fri Jun 01 00:00:00 UTC 2018

Characterization of CD127^- CD25⁺⁺ Treg from human colostrum.

American journal of reproductive immunology (New York, N.Y. : 1989)

Cérbulo-Vázquez A,Hernández-Peláez G,Arriaga-Pizano LA,Bautista-Pérez P,Romero-Venado J,Flores-González JC,Figueroa-Damian R,Soriano-Becerril D,Mancilla-Herrera I

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Thu Feb 01 00:00:00 UTC 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Sat Sep 01 00:00:00 UTC 2018

Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells.

Cell reports

Kim C,Hu B,Jadhav RR,Jin J,Zhang H,Cavanagh MM,Akondy RS,Ahmed R,Weyand CM,Goronzy JJ

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Tue Nov 20 00:00:00 UTC 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

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Two separate effects contribute to regulatory T cell defect in systemic lupus erythematosus patients and their unaffected relatives.

Clinical and experimental immunology

Costa N,Marques O,Godinho SI,Carvalho C,Leal B,Figueiredo AM,Vasconcelos C,Marinho A,Moraes-Fontes MF,Gomes da Costa A,Ponte C,Campanilho-Marques R,Cóias T,Martins AR,Viana JF,Lima M,Martins B,Fesel C

47-1278 was used in Flow cytometry/Cell sorting to determine the origin and role of the SLE Treg phenotype in systemic lupus erythematosus.

Fri Sep 01 00:00:00 UTC 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Tue Dec 26 00:00:00 UTC 2017

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

Thu Jan 04 00:00:00 UTC 2018

Persistence of asthma requires multiple feedback circuits involving type 2 innate lymphoid cells and IL-33.

The Journal of allergy and clinical immunology

Christianson CA,Goplen NP,Zafar I,Irvin C,Good JT,Rollins DR,Gorentla B,Liu W,Gorska MM,Chu H,Martin RJ,Alam R

47-1278 was used in Flow cytometry/Cell sorting to present a critical network of feedback and feed-forward interactions between epithelial cells and ILC2s involved in maintaining chronic asthma.

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Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.

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Nausch N,Appleby LJ,Sparks AM,Midzi N,Mduluza T,Mutapi F

47-1278 was used in Flow cytometry/Cell sorting to study the epxression of Lin, CD45, CD127, CD294 and CD161 by group 2 innate lymphoid cells obtained from young helminth infected children.

Sun Mar 01 00:00:00 UTC 2015

High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.

Cancer research

Péguillet I,Milder M,Louis D,Vincent-Salomon A,Dorval T,Piperno-Neumann S,Scholl SM,Lantz O

47-1278 was used in Flow cytometry/Cell sorting to characterise a population of human differentiated effector CD4(+) T cells that is defined by low levels of the interleukin (IL)-2 and IL-7 receptors, showing that this population expands in various types of cancer and displays cytotoxic features, suggesting that they are involved in human antitumour response.

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Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells.

European journal of immunology

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Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

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Delta-like 1-mediated Notch signaling enhances the in vitro conversion of human memory CD4 T cells into FOXP3-expressing regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Mota C,Nunes-Silva V,Pires AR,Matoso P,Victorino RM,Sousa AE,Caramalho I

47-1278 was used in Flow cytometry/Cell sorting to investigate novel strategies to generate in vitro-induced Tregs.

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IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.

Journal of immunology (Baltimore, Md. : 1950)

Tang XZ,Jo J,Tan AT,Sandalova E,Chia A,Tan KC,Lee KH,Gehring AJ,De Libero G,Bertoletti A

47-1278 was used in Flow cytometry/Cell sorting to investigate the function of human mucosal-associated invariant T (MAIT) cells in the human liver and peripheral blood.

Mon Apr 01 00:00:00 UTC 2013

Long-term effects of alemtuzumab on regulatory and memory T-cell subsets in kidney transplantation.

Transplantation

Macedo C,Walters JT,Orkis EA,Isse K,Elinoff BD,Fedorek SP,McMichael JM,Chalasani G,Randhawa P,Demetris AJ,Zeevi A,Tan H,Shapiro R,Landsittel D,Lakkis FG,Metes D

47-1278 was used in Flow cytometry/Cell sorting to discover how memory and regulatory T-cell subsets are affected after treatment with lymphocyte-depleting antibodies.

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Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.

Nature immunology

Monticelli LA,Sonnenberg GF,Abt MC,Alenghat T,Ziegler CG,Doering TA,Angelosanto JM,Laidlaw BJ,Yang CY,Sathaliyawala T,Kubota M,Turner D,Diamond JM,Goldrath AW,Farber DL,Collman RG,Wherry EJ,Artis D

47-1278 was used in Flow cytometry/Cell sorting to demonstrate a critical role for lung ILCs in restoring airway epithelial integrity and tissue homeostasis after viral replication.

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Low telomerase activity in CD4+ regulatory T cells in patients with severe chronic GVHD after hematopoietic stem cell transplantation.

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Kawano Y,Kim HT,Matsuoka K,Bascug G,McDonough S,Ho VT,Cutler C,Koreth J,Alyea EP,Antin JH,Soiffer RJ,Ritz J

47-1278 was used in Flow cytometry/Cell sorting to examine telomere length and telomerase activity of Treg and conventional CD4(+) T cells in patients surviving >2 years following HSC transplant, showing low telomerase activity.

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Klarquist J,Denman CJ,Hernandez C,Wainwright DA,Strickland FM,Overbeck A,Mehrotra S,Nishimura MI,Le Poole IC

47-1278 was used in Flow cytometry to test the hypothesis that cutaneous depigmentation in human vitiligo, can progress in the absence of regulatory T cells.

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Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using CD127 monoclonal antibody (Product # 47-1278-42) in Flow Cytometry

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Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 UTC 2007

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 UTC 2007

生物信息学

蛋白别名: CD127; CD127 antigen; CDw127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

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基因别名: CD127; CDW127; IL-7R-alpha; IL7R; IL7RA; ILRA

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UniProt ID: (Human) P16871

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Entrez Gene ID: (Human) 3575

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功能

antigen binding interleukin-7 receptor activity protein binding transmembrane signal receptor

参与通路