Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE, eBioscience™

CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow)

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CD154 (CD40 Ligand) Antibody (12-1548-42) in Flow

Human peripheral blood cells unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) were stained with Anti-Human CD8a FITC (Product # 11-0088-42) and Anti-Human CD154 (CD40 Ligand) PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-1548-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Cynomolgus monkey, Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

24-31

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1963568

产品详细信息

Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.

Applications Reported: 24-31 has been reported for use in flow cytometric analysis.

Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of activated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 12-1548-42) in Flow Cytometry

Thu Jan 07 00:00:00 UTC 2021

T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus Species.

Journal of virology

Grifoni A,Voic H,Dhanda SK,Kidd CK,Brien JD,Buus S,Stryhn A,Durbin AP,Whitehead S,Diehl SA,De Silva AD,Balmaseda A,Harris E,Weiskopf D,Sette A

12-1548 was used in Flow cytometry/Cell sorting to test pools of epitopes derived from dengue (DENV), Zika (ZIKV), Japanese encephalitis (JEV), West Nile (WNV), and yellow fever (YFV) viruses by intracellular cytokine staining (ICS) using peripheral blood mononuclear cells (PBMCs) of individuals naturally exposed to DENV or immunized with DENV (TV005) or YF17D vaccine.

Mon May 04 00:00:00 UTC 2020

Application of Flow Cytometry in the Diagnostics Pipeline of Primary Immunodeficiencies Underlying Disseminated Talaromyces marneffei Infection in HIV-Negative Children.

Frontiers in immunology

Lee PP,Lao-Araya M,Yang J,Chan KW,Ma H,Pei LC,Kui L,Mao H,Yang W,Zhao X,Trakultivakorn M,Lau YL

12-1548-42 was used in Flow Cytometry to show that this simple and rapid diagnostic algorithm will be useful in guiding genetic studies for patients with disseminated talaromycosis requiring immunological investigations.

Mon Oct 26 00:00:00 UTC 2020

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.

Oncology letters

Zhang F,Zhu Y,Fan G,Hu S

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 12-1548-42) in Flow Cytometry

Mon Oct 01 00:00:00 UTC 2018

Targeted gene editing restores regulated CD40L function in X-linked hyper-IgM syndrome.

Blood

Hubbard N,Hagin D,Sommer K,Song Y,Khan I,Clough C,Ochs HD,Rawlings DJ,Scharenberg AM,Torgerson TR

12-1548 was used in Flow cytometry/Cell sorting to demonstrate the feasibility of engineered nuclease-directed gene repair to restore endogenously regulated CD40L, and the potential for its use in T-cell therapy for X-linked hyper-IgM syndrome syndrome.

Thu May 26 00:00:00 UTC 2016

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

12-1548 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 UTC 2014

Isolation of biologically-active exosomes from human plasma.

Journal of immunological methods

Muller L,Hong CS,Stolz DB,Watkins SC,Whiteside TL

12-1548 was used in Flow cytometry/Cell sorting to isolate and study the effects of exosomes present in human plasma on immune cells.

Mon Sep 01 00:00:00 UTC 2014

生物信息学

蛋白别名: CD154; CD40 antigen ligand; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

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基因别名: CD154; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP

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UniProt ID: (Human) P29965

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Entrez Gene ID: (Human) 959, (Cynomolgus monkey) 102138630

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功能

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding protein binding

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