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CD18 (LFA-1 beta) Antibody (BMS103BT) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC and CD18 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD18 clone R3.3 stains a subset of lymphocytes (pink), monocytes (orange) and granulocytes (blue).
产品信息
BMS103BT
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
R3.3
偶联物
形式
Liquid
浓度
10 µL/Test
纯化类型
Affinity chromatography
保存液
TBS, pH 7.2-7.4, with 1% BSA
内含物
0.02% sodium azide
保存条件
2-8°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10598214
产品详细信息
Description: BMS103 is also suitable as primary antibody in staining for FACS analysis, BMS103FI can be used for direct staining of cells, BMS103BT can be used in a 2-step procedure using the biotin-(strept)avidin system.
BMS103 can be used as a neutralizing agent.
Recommended CD18 is a 90-95 kDa type I transmembrane protein that heterodimerizes with the a chain of CD11a, CD11b and CD11c. The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions. CD18 is expressed broadly by human lymphocytes and monocytes. Peripheral blood granulocytes also express CD18, but at lower levels.
Applications Tested: Flow Cytometry.
Purity: >95%.
靶标信息
CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.
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生物信息学
蛋白别名: CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
基因别名: CD18; ITGB2; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7
UniProt ID: (Human) P05107
Entrez Gene ID: (Human) 3689
leukocyte migration involved in inflammatory response
phagocytosis
apoptotic process
inflammatory response
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
cell-cell signaling
aging
regulation of cell shape
natural killer cell activation
extracellular matrix organization
neutrophil chemotaxis
receptor internalization
heterotypic cell-cell adhesion
toll-like receptor 4 signaling pathway
endodermal cell differentiation
receptor clustering
endothelial cell migration
cellular extravasation
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
leukocyte migration
positive regulation of NF-kappaB transcription factor activity
cellular response to low-density lipoprotein particle stimulus
