Invitrogen

CD19 Monoclonal Antibody (MB19-1), Biotin, eBioscience™

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CD19 Antibody (13-0191-82) in Flow

Staining of C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg of Anti-Mouse CD19 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

13-0191-82

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看9篇已发表文章 9篇已发表文章

其他PubMed (Misc)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgA, kappa

分类

Monoclonal

类型

Antibody

克隆号

MB19-1

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466384

产品详细信息

Description: The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

Applications Reported: The MB19-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Scientific reports

Tran A,Scholtes C,Songane M,Champagne C,Galarneau L,Levasseur MP,Fodil N,Dufour CR,Giguère V,Saleh M

Published figure using CD19 monoclonal antibody (Product # 13-0191-82) in Immunohistochemistry (Paraffin)

Fri Jul 23 00:00:00 UTC 2021

Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment.

Cell stem cell

Yamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H

13-0191 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment.

Thu Apr 05 00:00:00 UTC 2018

Focal adhesion kinase (FAK) deficiency in mononuclear phagocytes alters murine breast tumor progression.

American journal of cancer research

Llewellyn RA,Gutknecht MF,Thomas KS,Conaway MR,Bouton AH

13-0191 was used in Flow cytometry/Cell sorting to determine that focal adhesion kinase expression in this cell population influences primary breast tumour initiation and growth.

Wed Sep 30 00:00:00 UTC 2020

Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.

Nature communications

Yi S,Zhai J,Niu R,Zhu G,Wang M,Liu J,Huang H,Wang Y,Jing X,Kang L,Song W,Shi Y,Tang H

13-0191 was used in Flow cytometry/Cell sorting to suggest that different lung antigen-presenting cells modulate lung cDC1-mediated eosinophil recruitment dynamically.

Mon Sep 24 00:00:00 UTC 2018

Acidic chitinase primes the protective immune response to gastrointestinal nematodes.

Nature immunology

Vannella KM,Ramalingam TR,Hart KM,de Queiroz Prado R,Sciurba J,Barron L,Borthwick LA,Smith AD,Mentink-Kane M,White S,Thompson RW,Cheever AW,Bock K,Moore I,Fitz LJ,Urban JF,Wynn TA

13-0191 was used in Flow cytometry/Cell sorting to use AMCase-deficient mice as a means to dissect the role of the enzyme in several models of helminth infection and type 2 cytokine-driven airway inflammation.

Sun May 01 00:00:00 UTC 2016

Inflammatory spleen monocytes can upregulate CD11c expression without converting into dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Drutman SB,Kendall JC,Trombetta ES

13-0191 was used in Magnetic cell separation to establish the contribution of inflammatory DCs (iDCs) and conventional DCs (cDCs) in response to anti-CD40 administration in mice.

Sun Apr 15 00:00:00 UTC 2012

Dendritic cells continue to capture and present antigens after maturation in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Drutman SB,Trombetta ES

13-0191 was used in Magnetic cell separation to study the dynamics of Ag capture and presentation in situ, showing that dendritic cells continue to capture and present antigens after maturation.

Sun Aug 15 00:00:00 UTC 2010

Differential geminin requirement for proliferation of thymocytes and mature T cells.

Journal of immunology (Baltimore, Md. : 1950)

Karamitros D,Kotantaki P,Lygerou Z,Veiga-Fernandes H,Pachnis V,Kioussis D,Taraviras S

13-0191 was used in Magnetic cell separation to gain insight into the in vivo role of Geminin in progenitor cell division and differentiation.

Mon Mar 01 00:00:00 UTC 2010

Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.

The Journal of clinical investigation

Bedoret D,Wallemacq H,Marichal T,Desmet C,Quesada Calvo F,Henry E,Closset R,Dewals B,Thielen C,Gustin P,de Leval L,Van Rooijen N,Le Moine A,Vanderplasschen A,Cataldo D,Drion PV,Moser M,Lekeux P,Bureau F

13-0191 was used in Flow cytometry/Cell sorting to reveal a crucial role for IMs in maintaining immune homeostasis in the respiratory tract.

Tue Dec 01 00:00:00 UTC 2009

E47 controls the developmental integrity and cell cycle quiescence of multipotential hematopoietic progenitors.

Journal of immunology (Baltimore, Md. : 1950)

Yang Q,Kardava L,St Leger A,Martincic K,Varnum-Finney B,Bernstein ID,Milcarek C,Borghesi L

13-0191 was used in Flow cytometry/Cell sorting to show E47 controls the developmental integrity and cell cycle quiescence of hematopoietic progenitors.

Sat Nov 01 00:00:00 UTC 2008

Msx2-interacting nuclear target protein (Mint) deficiency reveals negative regulation of early thymocyte differentiation by Notch/RBP-J signaling.

Proceedings of the National Academy of Sciences of the United States of America

Tsuji M,Shinkura R,Kuroda K,Yabe D,Honjo T

13-0191-82 was used in Flow Cytometry to o investigate where and how Notch regulates early T cell differentiation by using specific up-regulation of Notch signaling by Mint knockout.

Tue Jan 30 00:00:00 UTC 2007

Immature hematopoietic cells display selective requirements for adhesion- and degranulation-promoting adaptor protein in development and homeostatsis.

European journal of immunology

Dluzniewska J,Zou L,Harmon IR,Ellingson MT,Peterson EJ

13-0191-82 was used in Flow Cytometry to highlight the dynamic regulation of ADAP during T cell maturation and document expression patterns that suggest a possible role for ADAP in development of non-T hematopoietic lineages.

Thu Nov 01 00:00:00 UTC 2007

Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling.

Genes & development

Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV

13-0191 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors.