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CD207 (Langerin) Antibody (13-2075-82) in Flow
Intracellular staining of mouse CD207-transfected HEK-293T cells coexpressing eGFP with 0.015 µg of Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) (left) or 0.015 µg of Anti-Mouse CD207 (Langerin) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total cells were used for analysis.
产品信息
13-2075-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Hamster,
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
eBioL31
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_763456
产品详细信息
Description: The eBioL31 monoclonal antibody reacts with mouse CD207, also known as Langerin, which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). mouse CD207 is a 48 kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake. LC are located in the epidermis and upon activation, reduce CD207 expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines.
Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse CD207 cDNA. Preliminary data from mouse CD207 cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3/Transcription Factor Staining Bufer Set (Product # 00-5523). The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin. Differences in expression has been observed between mouse strains.
Applications Reported: This eBioL31 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistology staining of frozen tissue sections.
Applications Tested: This eBioL31 antibody has been tested by intracellular staining and flow cytometric analysis of CD207-transfected HEK-293T cells. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-type lectin domain family 4 member K; CD207; Langerhans cell specific c-type lectin; Langerin
基因别名: Cd207; Clec4k
UniProt ID: (Mouse) Q8VBX4
Entrez Gene ID: (Mouse) 246278
