Invitrogen

CD235a Monoclonal Antibody (JC159)

CD235a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD235a Antibody (MA5-12484) in IHC (P)

Immunohistochemistry analysis of Glycophorin A showing staining in the membrane of paraffin-embedded human spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperat... View More

CD235a Antibody in Flow Cytometry (Flow)

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产品信息

MA5-12484

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50-1:500

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:200-1:400

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

JC159

抗原

Immunogen Membrane preparation from splenic hairy cell leukemia

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10944059

靶标信息

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Restaining-based annotation for cancer histology segmentation to overcome annotation-related limitations among pathologists.

Patterns (New York, N.Y.)

Komura D,Onoyama T,Shinbo K,Odaka H,Hayakawa M,Ochi M,Herdiantoputri RR,Endo H,Katoh H,Ikeda T,Ushiku T,Ishikawa S

MA5-12484 was used in Immunohistochemistry to present SegPath-the largest annotation dataset (>10 times larger than publicly available annotations)-for the segmentation of hematoxylin and eosin (HE)-stained sections for eight major cell types in cancer tissue.

Fri Feb 10 00:00:00 UTC 2023

Molecular Background of Toxic-Substances-Induced Morphological Alterations in the Umbilical Cord Vessels and Fetal Red Blood Cells.

International journal of molecular sciences

Zahorán S,Márton Á,Dugmonits K,Chakraborty P,Khamit A,Hegyi P,Orvos H,Hermesz E

MA5-12484 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to show evidence for the decreased level of DNA-PKcs-positive nuclei in samples with smoking origin, which is associated with the impaired DNA repair system.

Thu Nov 24 00:00:00 UTC 2022

Sustained maternal smoking-associated changes in the physico-chemical properties of fetal RBC membranes might serve as early markers for vascular comorbidities.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Balogh G,Chakraborty P,Dugmonits KN,Péter M,Végh AG,Vígh L,Hermesz E

MA5-12484 was used in Flow cytometry/Cell sorting to reveal induced membrane stiffness, loss in intrinsic plastic activities and several abnormalities in their membrane-lipid composition, that could consequently result in perturbed hemodynamic flow movements.

Wed Apr 01 00:00:00 UTC 2020

Erythrocytic α-synuclein contained in microvesicles regulates astrocytic glutamate homeostasis: a new perspective on Parkinson's disease pathogenesis.

Acta neuropathologica communications

Sheng L,Stewart T,Yang D,Thorland E,Soltys D,Aro P,Khrisat T,Xie Z,Li N,Liu Z,Tian C,Bercow M,Matsumoto J,Zabetian CP,Peskind E,Quinn JF,Shi M,Zhang J

MA5-12484 was used in Western Blotting to demonstrate that erythrocyte-derived EVs isolated from Parkinson''s disease patients carried elevated levels of oligomeric α-syn, compared to those from healthy controls.

Wed Jul 08 00:00:00 UTC 2020

Transmission of α-synuclein-containing erythrocyte-derived extracellular vesicles across the blood-brain barrier via adsorptive mediated transcytosis: another mechanism for initiation and progression of Parkinson's disease?

Acta neuropathologica communications

Matsumoto J,Stewart T,Sheng L,Li N,Bullock K,Song N,Shi M,Banks WA,Zhang J

Published figure using CD235a monoclonal antibody (Product # MA5-12484) in Western Blot

Wed Sep 13 00:00:00 UTC 2017

Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates.

British journal of haematology

Dugmonits KN,Ferencz Á,Zahorán S,Lázár R,Talapka P,Orvos H,Hermesz E

MA5-12484 was used in Immunocytochemistry-immunoflourescence to reveal the consequences of multiple pregnancies on the redox status of neonatal human red blood cells.

Thu Sep 01 00:00:00 UTC 2016

Histopathological, immunophenotypic and clinical particularities and evolution of a case of hepatosplenic T-cell lymphoma in transformation to leukemia.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Benedek Lázár E,Köpeczi JB,Tunyogi AB,Kakucs E,Horváth E,Turcu M,Benedek I

MA5-12484 was used in immunohistochemistry to report on a case of hepatosplenic T-cell lymphoma with leukemic transformation

Wed Jul 02 00:00:00 UTC 2014

生物信息学

蛋白别名: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha

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基因别名: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2

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UniProt ID: (Human) P02724

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Entrez Gene ID: (Human) 2993, (Rat) 688972

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功能

virus receptor activity identical protein binding

参与通路

viral entry into host cell