Invitrogen

CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), FITC, eBioscience™

CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)

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CD235a (Glycophorin A) Antibody (11-9987-82) in Flow

Staining of normal human whole blood with Mouse IgG2b kappa Isotype Control FITC (Product # 11-4732-42) (blue histogram) or Anti-Human CD235a (Glycophorin a) FITC (purple histogram). Cells in the erythrocyte gate were used for analysis.

CD235a (Glycophorin A) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

产品信息

11-9987-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.03 µg/test

查看35篇已发表文章 35篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HIR2 (GA-R2)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465477

产品详细信息

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.

Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Jan 31 00:00:00 UTC 2023

Autophagy regulated by the HIF/REDD1/mTORC1 signaling is progressively increased during erythroid differentiation under hypoxia.

Frontiers in cell and developmental biology

Li J,Quan C,He YL,Cao Y,Chen Y,Wang YF,Wu LY

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Sep 13 00:00:00 UTC 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

11-9987-82 was used in Flow Cytometry to pave the way for LSC-directed disease monitoring and therapy in this disease.

Mon Feb 01 00:00:00 UTC 2021

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

11-9987-82 was used in Flow Cytometry to pave the way for LSC-directed disease monitoring and therapy in this disease.

Mon Feb 01 00:00:00 UTC 2021

Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

Frontiers in cell and developmental biology

García-Montolio M,Ballaré C,Blanco E,Gutiérrez A,Aranda S,Gómez A,Kok CH,Yeung DT,Hughes TP,Vizán P,Di Croce L

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue May 18 00:00:00 UTC 2021

Negative Regulation of Erythroid Differentiation via the CBX8-TRIM28 Axis.

Molecules and cells

Kim HJ,Park JW,Kang JY,Seo SB

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Sat Jul 31 00:00:00 UTC 2021

Iron control of erythroid microtubule cytoskeleton as a potential target in treatment of iron-restricted anemia.

Nature communications

Goldfarb AN,Freeman KC,Sahu RK,Elagib KE,Holy M,Arneja A,Polanowska-Grabowska R,Gru AA,White Z,Khalil S,Kerins MJ,Ooi A,Leitinger N,Luckey CJ,Delehanty LL

11-9987 was used in Flow cytometry/Cell sorting to identify a cytoskeletal component of erythroid iron restriction and demonstrate potential for its therapeutic targeting in ACDI.

Fri Mar 12 00:00:00 UTC 2021

Iron control of erythroid microtubule cytoskeleton as a potential target in treatment of iron-restricted anemia.

Nature communications

Goldfarb AN,Freeman KC,Sahu RK,Elagib KE,Holy M,Arneja A,Polanowska-Grabowska R,Gru AA,White Z,Khalil S,Kerins MJ,Ooi A,Leitinger N,Luckey CJ,Delehanty LL

11-9987 was used in Flow cytometry/Cell sorting to identify a cytoskeletal component of erythroid iron restriction and demonstrate potential for its therapeutic targeting in ACDI.

Fri Mar 12 00:00:00 UTC 2021

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Fri Mar 19 00:00:00 UTC 2021

Extracellular vesicles are associated with C-reactive protein in sepsis.

Scientific reports

Fendl B,Weiss R,Eichhorn T,Linsberger I,Afonyushkin T,Puhm F,Binder CJ,Fischer MB,Weber V

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Fri Mar 26 00:00:00 UTC 2021

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV.

Pathogens (Basel, Switzerland)

Bazié WW,Goyer B,Boucher J,Zhang Y,Planas D,Chatterjee D,Routy JP,Alary M,Ancuta P,Gilbert C

11-9987-82 was used in Flow Cytometry to investigate daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV-).

Sat Apr 24 00:00:00 UTC 2021

The immunoregulatory function of peripheral blood CD71⁺ erythroid cells in systemic-onset juvenile idiopathic arthritis.

Scientific reports

Kanemasa H,Ishimura M,Eguchi K,Tanaka T,Nanishi E,Shiraishi A,Goto M,Motomura Y,Ohga S

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Jul 13 00:00:00 UTC 2021

Optimizing extracellular vesicles' isolation from chronic lymphocytic leukemia patient plasma and cell line supernatant.

JCI insight

Elgamal S,Cocucci E,Sass EJ,Mo XM,Blissett AR,Calomeni EP,Rogers KA,Woyach JA,Bhat SA,Muthusamy N,Johnson AJ,Larkin KT,Byrd JC

11-9987-82 was used in Flow Cytometry to characterize current approaches used in isolating extracellular vesicle products and understand whether cell culture-conditioned media or complex biological fluids confound results.

Mon Aug 09 00:00:00 UTC 2021

A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.

Cell stem cell

Xi H,Langerman J,Sabri S,Chien P,Young CS,Younesi S,Hicks M,Gonzalez K,Fujiwara W,Marzi J,Liebscher S,Spencer M,Van Handel B,Evseenko D,Schenke-Layland K,Plath K,Pyle AD

11-9987-82 was used in Flow Cytometry to profile human embryonic, fetal and postnatal skeletal muscle tissues to develop a roadmap of muscle ontogeny.

Thu Jul 02 00:00:00 UTC 2020

Microglia promote glioblastoma via mTOR-mediated immunosuppression of the tumour microenvironment.

The EMBO journal

Dumas AA,Pomella N,Rosser G,Guglielmi L,Vinel C,Millner TO,Rees J,Aley N,Sheer D,Wei J,Marisetty A,Heimberger AB,Bowman RL,Brandner S,Joyce JA,Marino S

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Mon Aug 03 00:00:00 UTC 2020

Proteosomal degradation of NSD2 by BRCA1 promotes leukemia cell differentiation.

Communications biology

Park JW,Kang JY,Hahm JY,Kim HJ,Seo SB

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Fri Aug 21 00:00:00 UTC 2020

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Thu May 09 00:00:00 UTC 2019

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Jun 11 00:00:00 UTC 2019

Arsenic sulfide nanoformulation induces erythroid differentiation in chronic myeloid leukemia cells through degradation of BCR-ABL.

International journal of nanomedicine

Wang T,Wen T,Li H,Han B,Hao S,Wang C,Ma Q,Meng J,Liu J,Xu H

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Fri Oct 18 00:00:00 UTC 2019

Genetic programming of macrophages generates an in vitro model for the human erythroid island niche.

Nature communications

Lopez-Yrigoyen M,Yang CT,Fidanza A,Cassetta L,Taylor AH,McCahill A,Sellink E,von Lindern M,van den Akker E,Mountford JC,Pollard JW,Forrester LM

11-9987 was used in Flow cytometry/Cell sorting to develop an in vitro system to model the human erythroblastic island niche.

Wed Feb 20 00:00:00 UTC 2019

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Mon Sep 10 00:00:00 UTC 2018

Mechanisms of establishment and functional significance of DNA demethylation during erythroid differentiation.

Blood advances

Bartholdy B,Lajugie J,Yan Z,Zhang S,Mukhopadhyay R,Greally JM,Suzuki M,Bouhassira EE

11-9987 was used in Flow cytometry/Cell sorting to study the mechanisms of establishment and functional significance of DNA demethylation during erythroid differentiation.

Tue Aug 14 00:00:00 UTC 2018

Acquired Resistance to FGFR Inhibitor in Diffuse-Type Gastric Cancer through an AKT-Independent PKC-Mediated Phosphorylation of GSK3β.

Molecular cancer therapeutics

Lau WM,Teng E,Huang KK,Tan JW,Das K,Zang Z,Chia T,Teh M,Kono K,Yong WP,Shabbir A,Tay A,Phua NS,Tan P,Chan SL,So JBY

Mon Jan 01 00:00:00 UTC 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

11-9987 was used in Flow cytometry/Cell sorting to investigate the role of KLF1 in maturation of red blood cells.

Sat Apr 01 00:00:00 UTC 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

11-9987 was used in Flow cytometry/Cell sorting to investigate the role of KLF1 in maturation of red blood cells.

Sat Apr 01 00:00:00 UTC 2017

Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Feb 09 00:00:00 UTC 2016

Platelets confound the measurement of extracellular miRNA in archived plasma.

Scientific reports

Mitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Sep 13 00:00:00 UTC 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Thu Jun 25 00:00:00 UTC 2015

Allele-specific analysis of DNA replication origins in mammalian cells.

Nature communications

Bartholdy B,Mukhopadhyay R,Lajugie J,Aladjem MI,Bouhassira EE

11-9987 was used in Flow cytometry/Cell sorting to delineate the mechanisms that control the location and timing of firing of replication origins.

Tue May 19 00:00:00 UTC 2015

Altered translation of GATA1 in Diamond-Blackfan anemia.

Nature medicine

Ludwig LS,Gazda HT,Eng JC,Eichhorn SW,Thiru P,Ghazvinian R,George TI,Gotlib JR,Beggs AH,Sieff CA,Lodish HF,Lander ES,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Jul 01 00:00:00 UTC 2014

EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.

Stem cells (Dayton, Ohio)

Zheng WW,Dong XM,Yin RH,Xu FF,Ning HM,Zhang MJ,Xu CW,Yang Y,Ding YL,Wang ZD,Zhao WB,Tang LJ,Chen H,Wang XH,Zhan YQ,Yu M,Ge CH,Li CY,Yang XM

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Fri Aug 01 00:00:00 UTC 2014

Cutting-edge analysis of extracellular microparticles using ImageStream(X) imaging flow cytometry.

Scientific reports

Headland SE,Jones HR,D'Sa AS,Perretti M,Norling LV

11-9987 was used in Immunocytochemistry to investigate methods to quantify microparticle levels.

Tue Jun 10 00:00:00 UTC 2014

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Immunofluorescence

Thu May 02 00:00:00 UTC 2013

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Tue Oct 01 00:00:00 UTC 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Fri Jun 07 00:00:00 UTC 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 11-9987-82) in Flow Cytometry

Thu Sep 29 00:00:00 UTC 2011

CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Landrigan A,Wong MT,Utz PJ

11-9987 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells.

Thu Sep 15 00:00:00 UTC 2011

Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells.

PloS one

Sjeklocha LM,Park CW,Wong PY,Roney MJ,Belcher JD,Kaufman DS,Vercellotti GM,Hebbel RP,Steer CJ

11-9987 was used in Flow cytometry/Cell sorting to investigate methods for efficient delivery of tightly regulated and stably expressed gene products, showing erythroid-specific expression of β-globin using the Sleeping Beauty transposon system.

Mon May 21 00:00:00 UTC 2012

Binding and uptake of H-ferritin are mediated by human transferrin receptor-1.

Proceedings of the National Academy of Sciences of the United States of America

Li L,Fang CJ,Ryan JC,Niemi EC,Lebrón JA,Björkman PJ,Arase H,Torti FM,Torti SV,Nakamura MC,Seaman WE

11-9987 was used in Flow cytometry/Cell sorting to demonstrate that TfR1 can bind HFt as well as Tf.

Tue Feb 23 00:00:00 UTC 2010

生物信息学

蛋白别名: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha

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基因别名: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2

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UniProt ID: (Human) P02724

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Entrez Gene ID: (Human) 2993

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功能

virus receptor activity identical protein binding

参与通路

viral entry into host cell