Invitrogen

CD25 Monoclonal Antibody (OX39), PE, eBioscience™

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CD25 Antibody (12-0390-82) in Flow

Staining of rat splenocytes with Anti-Rat CD4 FITC (Product # 11-0040-82) and 0.06 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.06 µg of Anti-Rat CD25 PE (right). Total viable cells were used for analysis.

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产品信息

12-0390-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OX39

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1582268

产品详细信息

Description: The OX39 monoclonal antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineages as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

The OX39 clone has been shown to deplete CD25 cells in vivo.

Applications Reported: This OX39 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX39 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

miR-126 ameliorates multiple organ dysfunction in septic rats by regulating the differentiation of Th17/Treg.

Molecular biology reports

Zou Q,Liu C,Hu N,Wang W,Wang H

12-0390-82 was used in Flow Cytometry to show that miR-126 can regulate the differentiation of Th17/Treg, change the infiltration of inflammatory factors in peripheral blood, lung, liver and kidney of septic rats, alleviate multiple organ dysfunction syndrome, and improve the organ function and prognosis of septic rats.

Fri Apr 01 00:00:00 UTC 2022

miR-126 ameliorates multiple organ dysfunction in septic rats by regulating the differentiation of Th17/Treg.

Molecular biology reports

Zou Q,Liu C,Hu N,Wang W,Wang H

Fri Apr 01 00:00:00 UTC 2022

Th17 Activation and Th17/Treg Imbalance in Prolonged Anterior Intraocular Inflammation after Ocular Alkali Burn.

International journal of molecular sciences

Yuan M,Qian X,Huang Y,Ma X,Duan F,Yang Y,Lou B,Lin X

12-0390-82 was used in Flow cytometry/Cell sorting to suggest that Th17 activation and Th17/Treg imbalance account for prolonged anterior intraocular inflammation after OAB.

Sat Jun 25 00:00:00 UTC 2022

Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis.

Mediators of inflammation

Li HB,Zhou Y,Zhao AH,Guo LL

12-0390 was used in Flow cytometry/Cell sorting to report that sepsis induced MVZ to inhibit CAP which underlies the inflammation storm.

Mon Aug 02 00:00:00 UTC 2021

Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis.

Mediators of inflammation

Li HB,Zhou Y,Zhao AH,Guo LL

12-0390 was used in Flow cytometry/Cell sorting to report that sepsis induced MVZ to inhibit CAP which underlies the inflammation storm.

Mon Aug 02 00:00:00 UTC 2021

Effect of Electroacupuncture at Zusanli (ST36) on Sepsis Induced by Cecal Ligation Puncture and Its Relevance to Spleen.

Evidence-based complementary and alternative medicine : eCAM

Xie DP,Zhou GB,Chen RL,Qin XL,Du JD,Zhang Y,Weng YN,Mai ST,Lai F,Han Y

12-0390 was used in Flow cytometry/Cell sorting to explore the effects of electroacupuncture (EA) at ST36, LI11, and ST25 on septic rats and investigate the role of the spleen in the treatment of EA at ST36.

Sun Apr 17 00:00:00 UTC 2022

Orthodontic treatment induces Th17/Treg cells to regulate tooth movement in rats with periodontitis.

Iranian journal of basic medical sciences

Ge N,Peng J,Yu L,Huang S,Xu L,Su Y,Chen L

Published figure using CD25 monoclonal antibody (Product # 12-0390-82) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

M1 Macrophage Derived Exosomes Aggravate Experimental Autoimmune Neuritis via Modulating Th1 Response.

Frontiers in immunology

Du T,Yang CL,Ge MR,Liu Y,Zhang P,Li H,Li XL,Li T,Liu YD,Dou YC,Yang B,Duan RS

12-0390 was used in Flow cytometry/Cell sorting to determine the effects of macrophage derived exosomes on the development of EAN in Lewis rats.

Tue Apr 13 00:00:00 UTC 2021

Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

International journal of molecular medicine

Yin M,Shen Z,Yang L,Zheng W,Song H

12-0390 was used in Flow cytometry/Cell sorting to investigate whether bone marrow mesenchymal stem cells modified by CXC-chemokine receptor type 3 and haem oxygenase-1 genes can repair damaged intestinal epithelial cells in vitro.

Wed May 01 00:00:00 UTC 2019

Placenta‑derived mesenchymal stem cells improve airway hyperresponsiveness and inflammation in asthmatic rats by modulating the Th17/Treg balance.

Molecular medicine reports

Li Y,Li H,Cao Y,Wu F,Ma W,Wang Y,Sun S

Published figure using CD25 monoclonal antibody (Product # 12-0390-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Adipose-derived mesenchymal stem cells suppress of acute rejection in small bowel transplantation.

Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association

Zhang Y,Meng Q,Zhang Y,Chen X,Wang Y

Mon Nov 05 00:00:00 UTC 2018

Proteomic changes of CD4⁺/CD25⁺/forkhead box p3⁺ regulatory T cells in a 30-day rat model of sepsis survival.

Experimental and therapeutic medicine

Jiao Y,Tan S,Xiong J

12-0390 was used in Flow cytometry/Cell sorting to explain the mechanism by which regulatory T-cells (Tregs) naturally recover and indicate that Tregs in the blood and spleen vary.

Fri Dec 01 00:00:00 UTC 2017

The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model.

BMC complementary and alternative medicine

Xie L,Zhong X,Liu D,Liu L,Xia Z

12-0390 was used in Flow cytometry/Cell sorting to show that freeze-dried powder from G. lucidum mycelia could potentially be used to treat recurrent oral ulceration.

Fri Dec 01 00:00:00 UTC 2017

Effect of cold stress on immunity in rats.

Experimental and therapeutic medicine

Hu GZ,Yang SJ,Hu WX,Wen Z,He D,Zeng LF,Xiang Q,Wu XM,Zhou WY,Zhu QX

12-0390 was used in Flow cytometry/Cell sorting to determine the effect of a sudden ambient temperature drop on the levels of stress hormones and T-lymphocyte cytokines in the plasma of mice.

Fri Jan 01 00:00:00 UTC 2016

Deficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis.

Mediators of inflammation

Oliveira L,Correia A,Cristina Costa A,Guerra-Gomes S,Ferreirinha F,Magalhães-Cardoso MT,Vilanova M,Correia-de-Sá P

12-0390 was used in Flow cytometry/Cell sorting to investigate the role of ecto-5'-nucleotidase/CD73 in a rat model of experimental autoimmune myasthenia gravis.

Mon Jan 11 00:00:00 UTC 2016

Magnetic resonance imaging investigation of macrophages in acute cardiac allograft rejection after heart transplantation.

Circulation. Cardiovascular imaging

Wu YL,Ye Q,Eytan DF,Liu L,Rosario BL,Hitchens TK,Yeh FC,Rooijen van N,Ho C

12-0390 was used in Flow cytometry/Cell sorting to indicate that macrophages are essential in acute cardiac allograft rejection.

Fri Nov 01 00:00:00 UTC 2013

A.actinomycetemcomitans-induced periodontal disease promotes systemic and local responses in rat periodontium.

Journal of clinical periodontology

Bezerra Bde B,Andriankaja O,Kang J,Pacios S,Bae HJ,Li Y,Tsiagbe V,Schreiner H,Fine DH,Graves DT

12-0390 was used in Flow cytometry/Cell sorting to link A. actinomycetemcomitans infection with important characteristics of periodontal destruction.

Sun Apr 01 00:00:00 UTC 2012

Aggregatibacter actinomycetemcomitans infection enhances apoptosis in vivo through a caspase-3-dependent mechanism in experimental periodontitis.

Infection and immunity

Kang J,de Brito Bezerra B,Pacios S,Andriankaja O,Li Y,Tsiagbe V,Schreiner H,Fine DH,Graves DT

12-0390 was used in Flow cytometry/Cell sorting to investigate whether diabetes aggravates periodontal destruction induced by A. actinomycetemcomitans infection, showing that infection enhances apoptosis in vivo through a caspase-3-dependent mechanism.

Fri Jun 01 00:00:00 UTC 2012

生物信息学

蛋白别名: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; sIL 2R; soluble IL 2 receptor

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基因别名: Il2ra; IL2RAC

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UniProt ID: (Rat) P26897

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Entrez Gene ID: (Rat) 25704

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功能

interleukin-2 receptor activity drug binding interleukin-2 binding transmembrane signal receptor

参与通路