Invitrogen

CD25 Monoclonal Antibody (eBio7D4 (7D4)), Biotin, eBioscience™

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CD25 Antibody (13-0252-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD4 APC (Product # 17-0042-82) and 0.06 µg of Rat IgM kappa Isotype Control Biotin (Product # 13-4341-81) (left) or 0.06 µg of Anti-Mouse CD25 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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产品信息

13-0252-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看7篇已发表文章 7篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgM, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio7D4 (7D4)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_891428

产品详细信息

Description: The eBio7D4 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (Product # 16-0251) is recommended for in vivo studies.

Purity is > 95% IgG2a as determined by SDS-gel electrophoresis. Endotoxin: <0.5 EU/µg

Applications Tested: This eBio7D4 (7D4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.

JCI insight

Wilson CS,Stocks BT,Hoopes EM,Rhoads JP,McNew KL,Major AS,Moore DJ

Published figure using CD25 monoclonal antibody (Product # 13-0252-82) in Flow Cytometry

Fri Oct 08 00:00:00 UTC 2021

Type 1 Innate Lymphoid Cells Protect Mice from Acute Liver Injury via Interferon-γ Secretion for Upregulating Bcl-xL Expression in Hepatocytes.

Immunity

Nabekura T,Riggan L,Hildreth AD,O'Sullivan TE,Shibuya A

13-0252 was used in Flow cytometry/Cell sorting to suggest that ILC1s are critical for tissue protection during acute liver injury.

Tue Jan 14 00:00:00 UTC 2020

Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice.

Journal of translational autoimmunity

Jackson-Thompson BM,Torrero M,Mitre BK,Long J,Packiam M,Mitre E

Published figure using CD25 monoclonal antibody (Product # 13-0252-82) in Flow Cytometry

Mon Sep 28 00:00:00 UTC 2020

Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy.

Nature communications

Khattar E,Maung KZY,Chew CL,Ghosh A,Mok MMH,Lee P,Zhang J,Chor WHJ,Cildir G,Wang CQ,Mohd-Ismail NK,Chin DWL,Lee SC,Yang H,Shin YJ,Nam DH,Chen L,Kumar AP,Deng LW,Ikawa M,Gunaratne J,Osato M,Tergaonkar V

13-0252-82 was used in Flow Cytometry to provide the genetic evidence that mammalian Rap1 plays a major role in hematopoietic stem cell survival, oncogenesis and response to chemotherapy.

Fri Dec 13 00:00:00 UTC 2019

Differential Roles of IL-2 Signaling in Developing versus Mature Tregs.

Cell reports

Fan MY,Low JS,Tanimine N,Finn KK,Priyadharshini B,Germana SK,Kaech SM,Turka LA

13-0252 was used in Flow cytometry/Cell sorting to study the differential roles of IL-2 signalling in developing versus mature regulatory T cells.

Tue Oct 30 00:00:00 UTC 2018

Partial depletion of regulatory T cells does not influence the inflammation caused by high dose hemi-body irradiation.

PloS one

Ma S,Richardson JA,Bitmansour A,Solberg TD,Pidikiti R,Song K,Stojadinovic S,Vitetta ES,Meyer JJ

13-0252 was used in Flow cytometry/Cell sorting to investigate the effects of partial depletion of regulatory T cells alongside ionizing radiation on inflammation and tissue injury in mice.

Mon Jul 29 00:00:00 UTC 2013

Donor B cells in transplants augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like chronic graft-versus-host disease.

Journal of immunology (Baltimore, Md. : 1950)

Young JS,Wu T,Chen Y,Zhao D,Liu H,Yi T,Johnston H,Racine J,Li X,Wang A,Todorov I,Zeng D

13-0252 was used in Flow cytometry/Cell sorting to investigate the mechanisms whereby donor B cells augment chronic graft-versus-host disease (cGVHD) pathogenesis, showing that B cells augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like cGVHD.

Sun Jul 01 00:00:00 UTC 2012

Hepatic stellate cells function as regulatory bystanders.

Journal of immunology (Baltimore, Md. : 1950)

Ichikawa S,Mucida D,Tyznik AJ,Kronenberg M,Cheroutre H

13-0252 was used in Magnetic cell separation to suggest that HSC can function as regulatory bystanders, and therefore, by promoting Tregs and suppressing Th17 differentiation, they might represent key players in the mechanism that drives liver-induced tolerance.

Sun May 15 00:00:00 UTC 2011

生物信息学

蛋白别名: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; p55 chain; sIL 2R; soluble IL 2 receptor

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基因别名: CD25; Il2r; Il2ra; Ly-43

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UniProt ID: (Mouse) P01590

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Entrez Gene ID: (Mouse) 16184

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功能

interleukin-2 receptor activity protein binding drug binding interleukin-2 binding transmembrane signal receptor

参与通路