Invitrogen

CD253 (TRAIL) Monoclonal Antibody (RIK-2), PE, eBioscience™

CD253 (TRAIL) Antibody in Flow Cytometry (Flow)

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CD253 (TRAIL) Antibody (12-9927-42) in Flow

hTrail/2PK3 cells were stained with Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (blue histogram) or CD253 Monoclonal Antibody, PE (purple histogram). Viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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产品信息

12-9927-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

RIK-2

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10557246

产品详细信息

Description: The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not expressed by resting human cells but has been shown to be induced under certain activation conditions. Iin addition it is expressed on several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis.

Applications Reported: The RIK-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RIK-2 antibody has been pre-titrated and tested by flow cytometric analysis of human TRAIL transfected cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Phase I clinical trial combining imatinib mesylate and IL-2: HLA-DR⁺ NK cell levels correlate with disease outcome.

Oncoimmunology

Chaput N,Flament C,Locher C,Desbois M,Rey A,Rusakiewicz S,Poirier-Colame V,Pautier P,Le Cesne A,Soria JC,Paci A,Rosenzwajg M,Klatzmann D,Eggermont A,Robert C,Zitvogel L

12-9927 was used in Flow cytometry/Cell sorting to show that the combination of IM and IL-2 promoted rapid expansion of HLA-DR+NK cells and increased the CD4+:CD8+T-cell ratio.

Fri Feb 01 00:00:00 UTC 2013

Inability of plasmacytoid dendritic cells to directly lyse HIV-infected autologous CD4+ T cells despite induction of tumor necrosis factor-related apoptosis-inducing ligand.

Journal of virology

Chehimi J,Papasavvas E,Tomescu C,Gekonge B,Abdulhaqq S,Raymond A,Hancock A,Vinekar K,Carty C,Reynolds G,Pistilli M,Mounzer K,Kostman J,Montaner LJ

12-9927 was used in Flow cytometry/Cell sorting to investigate the function of plasmacytoid dendritic cells in chronic HIV-1 infection.

Mon Mar 01 00:00:00 UTC 2010

TRAIL death receptor-4, decoy receptor-1 and decoy receptor-2 expression on CD8+ T cells correlate with the disease severity in patients with rheumatoid arthritis.

BMC musculoskeletal disorders

Bisgin A,Terzioglu E,Aydin C,Yoldas B,Yazisiz V,Balci N,Bagci H,Gorczynski RM,Akdis CA,Sanlioglu S

12-9927 was used in Flow cytometry/Cell sorting to study disease severity in patients with rheumatoid arthritis via TRAIL death receptor-4, decoy receptor-1 and decoy receptor-2 expression on CD8+ T cells.

Fri Aug 27 00:00:00 UTC 2010

Regulation of TNF-related apoptosis-inducing ligand on primary CD4+ T cells by HIV-1: role of type I IFN-producing plasmacytoid dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

Herbeuval JP,Hardy AW,Boasso A,Anderson SA,Dolan MJ,Dy M,Shearer GM

12-9927 was used in Flow cytometry/Cell sorting to elucidate the mechanism by which HIV-1 induces TRAIL expression on primary CD4+ T-cells.

Tue Sep 27 00:00:00 UTC 2005

生物信息学

蛋白别名: Apo-2 ligand; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Protein TRAIL; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; TRAIL protein; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; Tumor necrosis factor ligand superfamily member 10

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基因别名: Apo-2L; APO2L; CD253; TL2; TNFSF10; TNLG6A; TRAIL

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UniProt ID: (Human) P50591

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Entrez Gene ID: (Human) 8743

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功能

receptor binding cytokine activity tumor necrosis factor receptor binding protein binding metal ion binding

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