Invitrogen

CD28 Monoclonal Antibody (37.51), PE, eBioscience™

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CD28 Antibody (12-0281-82) in Flow

Swiss Webster mouse splenocytes were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0031-82) and 0.25 µg of Armenian Hamster IgG Isotype Control, PE (Product # 12-4888-83) (left) or 0.25 µg of CD28 Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

Product Image Gallery

CD28 Antibody — FIG 3 Differential effect of IL-7 and anti-PD-1 on CD28 in splenic and peripheral node T cells (A) Representative flow cytometric findings for CD28 expression on splenic CD3+CD8+ T cells. The percentage of CD3+CD8+ splenocytes that were positive for CD28 expression was greater in septic mice treated with IL-7 and combination of IL-7 and anti-PD-1 than mice treated with saline and anti-PD-1. (B) Representative flow cytometry findings for CD28 expression on peripheral lymph node CD3+CD4+ T cells. The percentage of CD3+CD4+ peripheral node T cells was increased in septic mice treated with PD-1 and combination of IL-7 and anti-PD-1 compared to mice treated with saline or IL-7. (C) These dot plots show percent positivity of CD28 expression on splenic CD3+CD4+ and CD3+CD8+ T cells. Values represent results from two separate experiments. The mean value of CD28 positivity on CD3+CD4+ T cells was more than 90% in all groups. IL-7 and combination of IL-7 and anti-PD-1 increased percentage of CD28 expression on CD3+CD8+ T cells compared with anti-PD-1. (D) These dot plots show percent positivity of CD28 expression on peripheral lymph node CD3+CD4+ and CD3+CD8+ T cells. Values represent represents from two separate experiments. Anti-PD-1 increased CD28 expression on CD3+CD4+ and IL-7 increased that on CD3+CD8+ T cells more frequently than saline. In particular, the additive effect of combination of IL-7 and anti-PD-1 was found in CD3+CD4+ and CD3+CD8+ T cells. IL-7, interleukin 7; aPD-1, {TM}

产品信息

12-0281-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看14篇已发表文章 14篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

37.51

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465621

产品详细信息

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

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Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

12-0281 was used in Flow cytometry/Cell sorting to show the phenomenon of brown adipose tissue 'whitening' in old mice, induced by IFN-γ secretion by senescent T cells.

Tue Mar 23 00:00:00 UTC 2021

Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-γ.

Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

12-0281 was used in Flow cytometry/Cell sorting to show the phenomenon of brown adipose tissue 'whitening' in old mice, induced by IFN-γ secretion by senescent T cells.

Tue Mar 23 00:00:00 UTC 2021

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

Published figure using CD28 monoclonal antibody (Product # 12-0281-82) in Flow Cytometry

Wed Sep 01 00:00:00 UTC 2021

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD28 monoclonal antibody (Product # 12-0281-82) in Flow Cytometry

Thu Sep 06 00:00:00 UTC 2018

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

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Published figure using CD28 monoclonal antibody (Product # 12-0281-82) in Flow Cytometry

Thu Sep 21 00:00:00 UTC 2017

The ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.

Nature communications

Xu Y,Zhao F,Qiu Q,Chen K,Wei J,Kong Q,Gao B,Melo-Cardenas J,Zhang B,Zhang J,Song J,Zhang DD,Zhang J,Fan Y,Li H,Fang D

Published figure using CD28 monoclonal antibody (Product # 12-0281-82) in Flow Cytometry

Fri Jul 15 00:00:00 UTC 2016

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

12-0281 was used in Flow cytometry/Cell sorting to investigate differences in immunomodulatory actions between IL-7 and anti-PD-1, to evaluate their potential use in the therapy of sepsis.

Wed Apr 01 00:00:00 UTC 2015

IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Journal of immunology (Baltimore, Md. : 1950)

Ding Y,Li J,Wu Q,Yang P,Luo B,Xie S,Druey KM,Zajac AJ,Hsu HC,Mountz JD

12-0281 was used in Flow cytometry/Cell sorting to investigate the role of germinal centres in autoimmunity, showing that IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

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Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.

Journal of immunology (Baltimore, Md. : 1950)

Driessens G,Zheng Y,Locke F,Cannon JL,Gounari F,Gajewski TF

12-0281 was used in Flow cytometry/Cell sorting to investigate the mechanism by which β-catenin inhibits T cell activation and cytokine secretion in a mouse model.

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Murine neonatal recent thymic emigrants are phenotypically and functionally distinct from adult recent thymic emigrants.

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12-0281 was used in Flow cytometry/Cell sorting to demonstrate that recent thymic emigrants from murine neonates and adults are phenotypically and functionally distinct.

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Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Lal G,Zhang N,van der Touw W,Ding Y,Ju W,Bottinger EP,Reid SP,Levy DE,Bromberg JS

12-0281 was used in Flow cytometry/Cell sorting to investigate Foxp3 as the master switch for Tregs, showing that expression is regulated by DNA methylation in Tregs.

Thu Jan 01 00:00:00 UTC 2009

In vivo induction of type 1-like regulatory T cells using genetically modified B cells confers long-term IL-10-dependent antigen-specific unresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Ahangarani RR,Janssens W,VanderElst L,Carlier V,VandenDriessche T,Chuah M,Weynand B,Vanoirbeek JA,Jacquemin M,Saint-Remy JM

12-0281 was used in Flow cytometry/Cell sorting to design a method for in vivo induction of Ag-specific Tregs, in BALB/c H-2d, that share characteristics with type 1 Tregs.

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High distribution of CD40 and TRAF2 in Th40 T cell rafts leads to preferential survival of this auto-aggressive population in autoimmunity.

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12-0281 was used in Flow cytometry/Cell sorting to study how CD40 may act as a switch between life and death promoting signals and NOD Th40 cells are poised for survival via this switch.

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Treatment with GITR agonistic antibody corrects adaptive immune dysfunction in sepsis.

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