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CD314 (NKG2D) Antibody (12-3140-82) in Flow
Staining of normal rat peripheral blood cells with Anti-Rat CD8a APC (Product # 17-0084-82) and 0.06 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.06 µg of Anti-Rat CD314 (NKG2D) PE (right). Cells in the lymphocyte gate were used for analysis.
产品信息
12-3140-82
产品规格
种属反应
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
11D5F4
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10804029
产品详细信息
Description: This 11D5F4 monoclonal antibody reacts with rat CD314, which is also known as NKG2D. This C-type lectin-like type II transmembrane protein is expressed on NK cells, subsets of T lymphocytes (e.g., CD8+ T cells and gammadelta T cells), NK T cells, and activated macrophages. The frequency of cells expressing NK2GD has been shown to vary among different rat strains. This receptor is involved in activating NK cell cytotoxicity, and has been reported to bind the MHC Class I-related ligands RAE-1L and RRLT to mediate allograft rejection in the rat.
The 11D5F4 antibody has been reported to block killing and IFN gamma secretion by NK cells co-cultured with dendritic cells.
Applications Reported: This 11D5F4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 11D5F4 antibody has been tested by flow cytometric analysis of normal rat peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; natural killer cell receptor; NK cell receptor D; NK lectin-like receptor; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NKLLR; NKR-P2; NKR-P2, ortholog of human NKG2D
基因别名: Klrk1; NKG2D; Nkrp2
UniProt ID: (Rat) O70215
Entrez Gene ID: (Rat) 24934
stimulatory C-type lectin receptor signaling pathway
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
natural killer cell activation
cell differentiation
positive regulation of myeloid dendritic cell activation
positive regulation of interferon-gamma production
positive regulation of apoptotic process
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of natural killer cell mediated cytotoxicity
defense response to Gram-positive bacterium
cellular response to lipopolysaccharide
